ABCMdb

PMID: 8572187

Howard M, DuVall MD, Devor DC, Dong JY, Henze K, Frizzell RA
Epitope tagging permits cell surface detection of functional CFTR.
Am J Physiol. 1995 Dec;269(6 Pt 1):C1565-76., [PubMed]

Sentences

No. Mutations Sentence Comment

241 ABCC7 p.Asn1303Lys A similar fate is expected for N1303K, which does not form a mature fully glycosylated protein. Login to comment 242 ABCC7 p.Gly551Asp Conversely, G551D CFTR also results in severe disease, but the protein appears to be fully glycosylated. Login to comment 243 ABCC7 p.Gly551Asp Immunofluorescence suggests colocalization of G551D with Na-K-ATPase at the plasma membrane of L cells (38). Login to comment 244 ABCC7 p.Gly551Asp ABCC7 p.Gly1349Asp ABCC7 p.Asn1303Lys We produced four tagged dgCFTR mutants that are associated with disease: AF508, N1303K, G551D, and G1349D, bearing the M2 epitope at position 901. Login to comment 251 ABCC7 p.Asp1370Asn ABCC7 p.Lys1250Met M2-901 CFIR 5 0 -1 mV C constructed two dgCFTR mutants that are not associated with disease, K1250M and D1370N, also bearing This is a technical study that identifies sites appropri- the M2 epitope at position 901. Login to comment 255 ABCC7 p.Gly551Asp ABCC7 p.Gly1349Asp ABCC7 p.Asp1370Asn ABCC7 p.Lys1250Met Cell surface expression of M2-901/CFTR has been observed in a expression of M2-901-labeled G551D, G1349D, K1250M, and D1370N dgCFTRs correlates with carbohydrate variety of cell types including HEp-2, BSC40, and addition (Fig &A-II). Login to comment 256 ABCC7 p.Asn1303Lys Conversely, M2-901-labeled AF508 MDCK (data not shown), so that the findings presented and N1303K dgCFTR were not detected in the plasma here are not restricted to HeLa cells. Login to comment 264 ABCC7 p.Asn1303Lys Hoescht stain confirmed the presence of cells in the field for AF508 and N1303K. Login to comment 265 ABCC7 p.Gly551Asp ABCC7 p.Gly1349Asp ABCC7 p.Asn1303Lys ABCC7 p.Asp1370Asn ABCC7 p.Lys1250Met A: G551D; B: G1349D; C: K1250M; D: D1370N; E: AF508; F: N1303K. Login to comment