PMID: 7542602

Gournier H, Pascolo S, Siegrist CA, Jehan J, Perarnau B, Garcia Z, Rose T, Neefjes J, Lemonnier FA
Restriction of self-antigen presentation to cytolytic T lymphocytes by mouse peptide pumps.
Eur J Immunol. 1995 Jul;25(7):2019-26., [PubMed]
Sentences
No. Mutations Sentence Comment
33 ABCB3 p.Leu180Gln
X
ABCB3 p.Leu180Gln 7542602:33:455
status: NEW
view ABCB3 p.Leu180Gln details
 H-2Kd molecules were immunoprecipitated as 2.4 Recombinant NP genes Paired synthetic oligonucleotides (Genset, Paris, France) corresponding to the 170-179 A3, Cw3, Kd, mutated Kd L174N, N176K, LlSOQ, mutated Cw3 N174L, K176N, mutated A3 N174L or mutated QIOb L174N peptide sequences (Table 1) were introduced in the NP coding region at the Bgl I1 site of the pTKNP Bgl I1 vector [28] providing the NP-A3, NP-Cw3, NP-Kd, mNP-Kd L174N, mNP-Kd N176K, mNP-Kd L180Q, mNP-Cw3 N174L, mNP-Cw3 K176N, mNP-A3 N174L and mNP-QlOhL174N constructs, which were verified by double-stranded DNA sequencing (Sequenase 7B, USB, Cleveland, OH). Login to comment