PMID: 24583180

Tsybovsky Y, Palczewski K
Expression, purification and structural properties of ABC transporter ABCA4 and its individual domains.
Protein Expr Purif. 2014 May;97:50-60. doi: 10.1016/j.pep.2014.02.010. Epub 2014 Feb 28., [PubMed]
Sentences
No. Mutations Sentence Comment
201 ABCA2 p.Gln2135Ser
X
ABCA2 p.Gln2135Ser 24583180:201:169
status: NEW
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 The resulting mutants containing from six down to two Cys showed unaltered expression levels and were refolded after purification from IB (Fig. 6b, left) except for the C2135S mutation construct that led to protein precipitation. Login to comment 218 ABCA4 p.Lys1978Met
X
ABCA4 p.Lys1978Met 24583180:218:77
status: NEW
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 To evaluate the contribution of these contaminants, we generated an inactive K1978M mutant [17] of his-CD2-strep and purified it by using exactly the same protocol, which resulted in similar protein yield and purity (data not shown). Login to comment