ABCMdb

PMID: 24378332

Aida K, Hayashi H, Inamura K, Mizuno T, Sugiyama Y
Differential roles of ubiquitination in the degradation mechanism of cell surface-resident bile salt export pump and multidrug resistance-associated protein 2.
Mol Pharmacol. 2014 Mar;85(3):482-91. doi: 10.1124/mol.113.091090. Epub 2013 Dec 30., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCB11 p.Tyr1311Ala The effect of UbDGG on BSEP internalization in these experiments was abrogated by treatment with chlorpromazine, an inhibitor of clathrin-mediated endocytosis, and the introduction of a Y1311A mutation into BSEP. Login to comment 28 ABCB11 p.Tyr1311Ala The cDNAs for human 3 FLAG-BSEP; 3 FLAG-BSEPY1311A , which incorporates a Y1311A mutation into 3 FLAG-BSEP; and MRP2 were cloned into pShuttle (Clontech, Mountain View, CA) (pShuttle-3 FLAG-BSEP and pShuttle-3 FLAG-BSEPY1311A ) and pcDNA3.1(1)-Neo (Invitrogen) (pcDNA3.1(1)-MRP2), respectively, as described previously (Hayashi et al., 2012a,b). Login to comment 97 ABCB11 p.Tyr1311Ala Elimination of the Inhibitory Effect of HA-UbDGG on the Internalization of 33 FLAG-BSEP by Treatment with Chlorpromazine and Introduction of a Y1311A Mutation. Login to comment 192 ABCB11 p.Asp482Gly ABCB11 p.Glu297Gly The cell surface-resident forms of BSEP with E297G and D482G, both of which are the most frequently found mutations in patients with progressive familial IC type 2, a genetic disorder caused by mutations in the BSEP gene, are highly ubiquitinated (Hayashi and Sugiyama, 2009). Login to comment