ABCMdb

PMID: 22454528

Emi Y, Yasuda Y, Sakaguchi M
A cis-acting five-amino-acid motif controls targeting of ABCC2 to the apical plasma membrane domain.
J Cell Sci. 2012 Jul 1;125(Pt 13):3133-43. Epub 2012 Mar 27., [PubMed]

Sentences

No. Mutations Sentence Comment

117 ABCC2 p.Val288Ala ABCC2 p.Leu287Ala ABCC2 p.Ser283Ala ABCC2 p.Gln284Ala Remarkably, cells expressing the S283A/Q284A and L287A/ V288A double substitution mutants exhibited mixed staining patterns. Login to comment 118 ABCC2 p.Val288Ala ABCC2 p.Leu287Ala ABCC2 p.Ser283Ala ABCC2 p.Gln284Ala Remarkably, cells expressing the S283A/Q284A and L287A/ V288A double substitution mutants exhibited mixed staining patterns. Login to comment 132 ABCC2 p.Leu287Ala ABCC2 p.Ser283Ala In particular, two point mutants, S283A and L287A, exhibited dual localization patterns in polarized HepG2 cells. Login to comment 134 ABCC2 p.Leu287Ala ABCC2 p.Ser283Ala In particular, two point mutants, S283A and L287A, exhibited dual localization patterns in polarized HepG2 cells. Login to comment 139 ABCC2 p.Ser283Glu ABCC2 p.Ser283Asp Notably, two mutants containing phosphomimetic substitutions at residue S283 , namely S283D and S283E, exhibited normal apical localization similar to that of the wild-type ABCC2 (Fig. 5). Login to comment 141 ABCC2 p.Ser283Glu ABCC2 p.Ser283Asp Notably, two mutants containing phosphomimetic substitutions at residue S283 , namely S283D and S283E, exhibited normal apical localization similar to that of the wild-type ABCC2 (Fig. 5). Login to comment 143 ABCC2 p.Gln284Ala As expected, FLAG-ABCC2 was readily seen at the apical side of MDCK cells and the Q284A mutant displayed normal Fig. 3. Login to comment 145 ABCC2 p.Gln284Ala As expected, FLAG-ABCC2 was readily seen at the apical side of MDCK cells and the Q284A mutant displayed normal Fig. 3. Login to comment 156 ABCC2 p.Ser283Ala ABCC2 p.Ser283Asp The S283A mutant lost its apical-specific steering activity and gave the mixed staining of the lateral side in addition to the apical side, whereas the S283D phosphomimetic substitution restored apical-specific localization. Login to comment 158 ABCC2 p.Ser283Ala ABCC2 p.Gln284Ala However, localization of S283A/Q284A and 279-283/A mutants looked mostly intracellular in MDCK cells. Login to comment 159 ABCC2 p.Ser283Ala ABCC2 p.Ser283Asp The S283A mutant lost its apical-specific steering activity and gave the mixed staining of the lateral side in addition to the apical side, whereas the S283D phosphomimetic substitution restored apical-specific localization. Login to comment 161 ABCC2 p.Ser283Ala ABCC2 p.Gln284Ala However, localization of S283A/Q284A and 279-283/A mutants looked mostly intracellular in MDCK cells. Login to comment 170 ABCC2 p.Ser283Ala FLAG-ABCC2 (S283A) exhibited a mixed localization pattern at the basolateral membranes and the apical vacuoles. Login to comment 173 ABCC2 p.Ser283Ala FLAG-ABCC2 (S283A) exhibited a mixed localization pattern at the basolateral membranes and the apical vacuoles. Login to comment 262 ABCC2 p.Ser283Glu ABCC2 p.Ser283Asp ABCC2 mutants containing phosphomimetic substitutions at S283 (S283D and S283E) showed normal apical localization, whereas mutants containing nonphosphorylatable substitutions at S283 were localized to both the apical and basolateral membranes. Login to comment 263 ABCC2 p.Ser283Glu ABCC2 p.Ser283Asp ABCC2 mutants containing phosphomimetic substitutions at S283 (S283D and S283E) showed normal apical localization, whereas mutants containing nonphosphorylatable substitutions at S283 were localized to both the apical and basolateral membranes. Login to comment 266 ABCC2 p.Ser283Glu ABCC2 p.Ser283Asp ABCC2 mutants containing phosphomimetic substitutions at S283 (S283D and S283E) showed normal apical localization, whereas mutants containing nonphosphorylatable substitutions at S283 were localized to both the apical and basolateral membranes. Login to comment