ABCC2 p.Gln284Ala
| Predicted by SNAP2: | A: N (82%), C: N (87%), D: N (87%), E: N (93%), F: N (78%), G: N (78%), H: N (93%), I: N (87%), K: N (93%), L: N (87%), M: N (93%), N: N (93%), P: N (82%), R: N (93%), S: N (93%), T: N (87%), V: N (87%), W: N (53%), Y: N (82%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, E: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, N: N, P: N, R: N, S: N, T: N, V: N, W: N, Y: N, |
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[hide] A cis-acting five-amino-acid motif controls target... J Cell Sci. 2012 Jul 1;125(Pt 13):3133-43. Epub 2012 Mar 27. Emi Y, Yasuda Y, Sakaguchi M
A cis-acting five-amino-acid motif controls targeting of ABCC2 to the apical plasma membrane domain.
J Cell Sci. 2012 Jul 1;125(Pt 13):3133-43. Epub 2012 Mar 27., [PMID:22454528]
Abstract [show]
ATP-binding cassette transporter isoform C2 (ABCC2) is exclusively targeted to the apical plasma membrane of polarized cells. Although apical localization of ABCC2 in hepatocytes is crucial for the biliary excretion of a variety of metabolites, the mechanism regulating its apical targeting is poorly understood. In the present study, an apical targeting signal was identified in the first cytoplasmic loop domain (CLD1) of ABCC2 in HepG2 cells. Overexpression of CLD1 significantly disturbed the apical targeting of FLAG-ABCC2 in a competitive manner, suggesting the presence of a saturable sorting machinery in HepG2 cells. Next, deletion analysis identified a potential targeting sequence within a 20-amino-acid long peptide (aa 272-291) of CLD1. Alanine scanning mutagenesis of this region in full-length ABCC2 further narrowed down the apical targeting determinant to five amino acids, S(283)QDAL(287). Of these, S(283) and L(287) were found to be conserved among vertebrate ABCC2 orthologs. Site-directed mutagenesis showed that both S(283) and L(287) were crucial for the targeting specificity of ABCC2. Introducing this apical targeting sequence into the corresponding region of ABCC1, an exclusively basolateral protein, caused the hybrid ABCC1 to partially localize in the apical membrane. Thus, the CLD1 of ABCC2 contains a novel apical sorting determinant, and a saturable sorting machinery is present in polarized HepG2 cells.
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No. Sentence Comment
118 Remarkably, cells expressing the S283A/Q284A and L287A/ V288A double substitution mutants exhibited mixed staining patterns.
X
ABCC2 p.Gln284Ala 22454528:118:39
status: NEW145 As expected, FLAG-ABCC2 was readily seen at the apical side of MDCK cells and the Q284A mutant displayed normal Fig. 3.
X
ABCC2 p.Gln284Ala 22454528:145:82
status: NEW161 However, localization of S283A/Q284A and 279-283/A mutants looked mostly intracellular in MDCK cells.
X
ABCC2 p.Gln284Ala 22454528:161:31
status: NEW117 Remarkably, cells expressing the S283A/Q284A and L287A/ V288A double substitution mutants exhibited mixed staining patterns.
X
ABCC2 p.Gln284Ala 22454528:117:39
status: NEW143 As expected, FLAG-ABCC2 was readily seen at the apical side of MDCK cells and the Q284A mutant displayed normal Fig. 3.
X
ABCC2 p.Gln284Ala 22454528:143:82
status: NEW158 However, localization of S283A/Q284A and 279-283/A mutants looked mostly intracellular in MDCK cells.
X
ABCC2 p.Gln284Ala 22454528:158:31
status: NEW