ABCMdb

PMID: 22384107

Ndiath MO, Sougoufara S, Gaye A, Mazenot C, Konate L, Faye O, Sokhna C, Trape JF
Resistance to DDT and pyrethroids and increased kdr mutation frequency in An. gambiae after the implementation of permethrin-treated nets in Senegal.
PLoS One. 2012;7(2):e31943. Epub 2012 Feb 22., [PubMed]

Sentences

No. Mutations Sentence Comment

5 ABCC8 p.Leu1014Ser ABCC8 p.Leu1014Phe Species and molecular forms as well as the presence of L1014F and L1014S kdr mutations were assessed by PCR. Login to comment 7 ABCC8 p.Leu1014Ser Findings: No specimen studied presented the L1014S mutation. Login to comment 8 ABCC8 p.Leu1014Phe During the longitudinal study, L1014F allelic frequency rose from 2.4% in year before the implementation of LLINs to 4.6% 0-12 months after and 18.7% 13-30 months after. Login to comment 22 ABCC8 p.Leu1014Phe Molecular identification of L1014F mutation indicated that target site resistance increased after the implementation of LLINs. Login to comment 80 ABCC8 p.Leu1014Ser ABCC8 p.Leu1014Phe Molecular identification and kdr genotyping In the subsample of mosquitoes used for bioassay and in adults sampled by HLC during the longitudinal study, detection of L1014F and L1014S kdr mutations (thereafter identified as kdr-w and kdr-e respectively) was performed by PCR [27,28]. Login to comment 91 ABCC8 p.Leu1014Ser gambiae s.l. From July 2006 to December 2010, no specimen with L1014S (kdr-e) mutation was identified. Login to comment 93 ABCC8 p.Leu1014Phe Before the implementation of LLINs, L1014F allelic frequency was low and not different when Figure 1. kdr mutation in An. Login to comment 96 ABCC8 p.Leu1014Phe gambiae with L1014F homozygote mutation (RR), heterozygote mutation (RS) or wild type (SS) sampled 24-13 months (n = 228) and 12-0 months (n = 99) before the implementation of long lasting insecticide-treated nets (LLINs) in July 2008, 0-12 months (n = 327) and 13-30 months after (n = 582). Login to comment 314 ABCC8 p.Leu1014Ser Djegbe I, Boussari O, Sidick A, Martin T, Ranson H, et al. (2011) Dynamics of insecticide resistance in malaria vectors in Benin: first evidence of the presence of L1014S kdr mutation in Anopheles gambiae from West Africa. Login to comment