ABCMdb

PMID: 22318656

Deo AK, Prasad B, Balogh L, Lai Y, Unadkat JD
Interindividual variability in hepatic expression of the multidrug resistance-associated protein 2 (MRP2/ABCC2): quantification by liquid chromatography/tandem mass spectrometry.
Drug Metab Dispos. 2012 May;40(5):852-5. Epub 2012 Feb 8., [PubMed]

Sentences

No. Mutations Sentence Comment

7 ABCC2 p.Val417Ile In contrast, the single nucleotide polymorphism 21214G>A (V417I; rs2273697) was associated with significantly higher hepatic MRP2 expression. Login to comment 85 ABCC2 p.Val417Ile ABCC2 p.Val1188Glu ABCC2 p.Tyr39Phe ABCC2 p.Leu849Arg Genotyping revealed polymorphisms for ABCC2 at the following sites: 1846AϾT(Y39F; rs927344), 21214GϾA(V417I; rs2273697), -24CϾT (rs717620), 36351TϾG(L849R; rs45494393), 53395TϾA(V1188E; rs17222723), 61606CϾT(I1324I; rs3740066), SNP-1 - - + - - - + - - + - - + - - - - - + - - - - - + - - + - - - - - - - - - + + - - + - - - - - - + + - SNP-2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - + - - - - - - - - - - - + - - - - - - - - + - SNP-3 - - - + + - - - - - - - - + + + - - + + - - + + - - - - - - + - - + + + - + - + - - - + - - - + + - - 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 HL102 HL103 HL105 HL106 HL111 HL112 HL113 HL114 HL115 HL119 HL127 HL129 HL132 HL137 HL139 HL152 HL155 HL157 HL167 HL168 HL171 HL172 Average HL104 HL109 HL120 HL126 HL128 HL133 HL135 HL142 HL144 HL150 HL151 HL153 HL156 HL158 HL159 HL162 HL163 HL165 Average HL125 HL136 HL138 HL146 Average HL143 HL145 HL147 HL149 HL166 Average HL141 HL140 MRP2protein(fmol/ugprotein) A Acute injury FaƩy FibroƟc Normal B FIG. 2. Login to comment 88 ABCC2 p.Val417Ile ABCC2 p.Cys1515Tyr SNP-1, SNP-2, and SNP-3 represent -24CϾT (rs717620), 68693GϾA(C1515Y; rs8187710) and 21214GϾA(V417I; rs2273697), respectively. Login to comment 90 ABCC2 p.Cys1515Tyr DEO ET AL. atUnivOfNCAcqSrvcsonOctober4,2012dmd.aspetjournals.orgDownloadedfrom 64260GϾT(V1430V; rs1137968), 67932CϾT(H1496H; rs8187707), and 68693GϾA(C1515Y; rs8187710) in the promoter, coding and noncoding regions. Login to comment 95 ABCC2 p.Val417Ile However, Meier et al. (2006) have shown that this SNP is associated with increased MRP2 expression. It is noteworthy that the SNP 21214GϾA (V417I; rs2273697; 16 heterozygotes, 2 homozygotes), which has not been reported to affect MRP2 expression, showed a significantly (P Ͻ 0.004) higher expression of MRP2 (1.87 Ϯ 0.77 fmol/␮g membrane protein; n ϭ 18) versus the wild-type livers (1.34 Ϯ 0.38 fmol/␮g membrane protein; n ϭ 27). Login to comment