ABCMdb

PMID: 21651761

Antonio-Nkondjio C, Fossog BT, Ndo C, Djantio BM, Togouet SZ, Awono-Ambene P, Costantini C, Wondji CS, Ranson H
Anopheles gambiae distribution and insecticide resistance in the cities of Douala and Yaounde (Cameroon): influence of urban agriculture and pollution.
Malar J. 2011 Jun 8;10:154., [PubMed]

Sentences

No. Mutations Sentence Comment

79 ABCC8 p.Leu1014Ser ABCC8 p.Leu1014Phe To screen for the presence of the kdr alleles (L1014F and L1014S) conferring resistance to DDT and pyrethroids, DNA extracted from individuals exposed to insecticide was tested using the Hot Ligation Oligonucleotide Assay (HOLA) of Lynd et al [26] and the TaqMan assay [27]. Login to comment 151 ABCC8 p.Leu1014Phe The L1014F allele was prevalent in all samples (Table 4). Login to comment 152 ABCC8 p.Leu1014Ser Only one specimen in Douala was found with the L1014S allele. Login to comment 197 ABCC8 p.Leu1014Ser ABCC8 p.Leu1014Phe gambiae molecular forms Genotypes Kdrw/Kdrw Kdrw/KdrE Kdrw/S S/S Total F(Kdr) P(HW) Douala M form 228 1 170 62 460 0.68 P = 0.002 S form - - 1 - 1 Yaoundé M form 79 0 124 115 318 0.44 P = 0.0003 S form - - 2 - 2 Total 307 1 296 177 781 S, wild type; Kdrw, L1014F substitution « West Africa"; KdrE, L1014S substitution "East Africa", Kdrw/S, heterozygote; P(HW), goodness of fit to Hardy Weinberg Equilibrium (significant if P < 0.05). Login to comment