ABCMdb

PMID: 19615439

Davidson H, Wilson A, Gray RD, Horsley A, Pringle IA, McLachlan G, Nairn AC, Stearns C, Gibson J, Holder E, Jones L, Doherty A, Coles R, Sumner-Jones SG, Wasowicz M, Manvell M, Griesenbach U, Hyde SC, Gill DR, Davies J, Collie DD, Alton EW, Porteous DJ, Boyd AC
An immunocytochemical assay to detect human CFTR expression following gene transfer.
Mol Cell Probes. 2009 Dec;23(6):272-80. Epub 2009 Jul 15., [PubMed]

Sentences

No. Mutations Sentence Comment

138 ABCC7 p.Gly551Asp Transfection of CF nasal epithelial cells Initially, nasal brushing samples from three CF subjects (2 DF508/DF508, 1 DF508/G551D) and one non-CF control were treated with 0.2 mg pG4-hCEFI-soCFTR2 (CFTR), 0.2 mg pG4-hCEFI- soLux (luciferase), or no DNA (control) (n ¼ 2 wells per sample/ treatment). Login to comment 141 ABCC7 p.Gly551Asp Transfection of CF nasal epithelial cells Initially, nasal brushing samples from three CF subjects (2 DF508/DF508, 1 DF508/G551D) and one non-CF control were treated with 0.2 mg pG4-hCEFI-soCFTR2 (CFTR), 0.2 mg pG4-hCEFI- soLux (luciferase), or no DNA (control) (n &#bc; 2 wells per sample/ treatment). Login to comment 162 ABCC7 p.Gly542* ABCC7 p.Pro67Leu TwotransfectedCF samples (1DF508/G542X,11585-1G>A/P67L) and three transfected non-CF samples were negative for plasmid-expressed CFTR. Login to comment 165 ABCC7 p.Gly542* ABCC7 p.Pro67Leu TwotransfectedCF samples (1DF508/G542X,11585-1G>A/P67L) and three transfected non-CF samples were negative for plasmid-expressed CFTR. Login to comment 215 ABCC7 p.Gly551Asp ABCC7 p.Ala455Glu ABCC7 p.Gly85Glu CF genotypes for nasal brushings were 15 DF508/DF508, 2 DF508/ND, 1 DF508/A455E, 1 DF508/G551D, 1 G85E/ND, 1 ND/ND (where 'ND` indicates that none of the 31 most common mutations was detected). Login to comment 217 ABCC7 p.Gly85Glu CF genotypes for bronchial brushings were 5 DF508/DF508, 1 G85E/ND and 1 ND/ND. Login to comment 218 ABCC7 p.Gly551Asp ABCC7 p.Ala455Glu ABCC7 p.Gly85Glu CF genotypes for nasal brushings were 15 DF508/DF508, 2 DF508/ND, 1 DF508/A455E, 1 DF508/G551D, 1 G85E/ND, 1 ND/ND (where 'ND` indicates that none of the 31 most common mutations was detected). Login to comment 220 ABCC7 p.Gly85Glu CF genotypes for bronchial brushings were 5 DF508/DF508, 1 G85E/ND and 1 ND/ND. Login to comment