ABCMdb

PMID: 19270533

Mathew G, Timm EA Jr, Sotomayor P, Godoy A, Montecinos VP, Smith GJ, Huss WJ
ABCG2-mediated DyeCycle Violet efflux defined side population in benign and malignant prostate.
Cell Cycle. 2009 Apr 1;8(7):1053-61. Epub 2009 Apr 30., 2009-04-01 [PubMed]

Sentences

No. Mutations Sentence Comment

27 ABCG2 p.Arg482Gly ABCG2 p.Arg482Gly ABCG2 p.Arg482Gly To establish the optimal dye concentration for DCV labeling of cultured human cell lines, MCF-7, a breast cancer cell line that expresses an endogenous ABCG2, and HEK 293, a human embryonic kidney cell line that lacks all ABC-family members that was transfected with human ABCG2 (R482G) cloned into pcDNA (HEK 293 (R482G)), were used as ABCG2-mediated SP containing control cell lines.11,22 A distinct DCV-efflux mediated SP was observed in flow cytometric analyses of MCF-7 and HEK 293 (R482G) cells (Fig. 1C and E), and the SP was eliminated when the cells were pre-incubated with FTC (Fig. 1D and F). Login to comment 44 ABCG2 p.Arg482Gly The SP is present in mouse bone marrow (A); in the MCF-7 cell line (C); and in the HEK 293 (R482G) cell line (E). Login to comment 45 ABCG2 p.Arg482Gly The loss of the SP population due to inhibition of the ABCG2 pump by FTC is observed in mouse bone marrow (B); in the MCF-7 cell line (D); and in the HEK 293 (R482G) cell line (F). Login to comment 91 ABCG2 p.Arg482Gly We confirmed that DCV is actively effluxed via ABCG2 by a subpopulation of mouse bone marrow cells that demonstrate the SP phenotype by the sensitivity of the efflux to inhibition by FTC.11 Analysis of the DCV profile in the HEK 293 (R482G) cell line pre-incubated with FTC confirmed that DCV efflux was mediated by the membrane transporter ABCG2. Login to comment 131 ABCG2 p.Arg482Gly HEK 293 cells stably transfected with the pcDNA empty vector, or vector containing cDNA coding for ABCG2 (R482G), were maintained in Eagle`s Minimum Essential Medium (MEM) media (Mediatech Inc., Herndon, VA) supplemented with 10% FBS (Mediatech Inc., Herndon, VA), 2 mM L-glutamine (Mediatech Inc., Herndon, VA), penicillin (100 U/ml) (Mediatech Inc., Herndon, VA), streptomycin (100 mg/ml) (Mediatech Inc., Herndon, VA), and 0.5 mg/ml G418 (Mediatech Inc., Herndon, VA). Login to comment 264 ABCG2 p.Arg482Gly Simon Hayward and HEK 293 (pcDNA) and HEK 293 (R482G) cells were a gift from Dr. Login to comment