ABCMdb

PMID: 19170766

Azuma Y, Takada M, Shin HW, Kioka N, Nakayama K, Ueda K
Retroendocytosis pathway of ABCA1/apoA-I contributes to HDL formation.
Genes Cells. 2009 Feb;14(2):191-204. Epub 2008 Jan 6., [PubMed]

Sentences

No. Mutations Sentence Comment

36 ABCA1 p.Trp590Ser A Tangier disease-type mutation (W590S) greatly reduced the cholesterol efflux activity of ABCA1(207HA), and lysine-to-methionine mutations (MM) in both of the two ATP-binding domains almost completely abolished activity (Fig. 1E), as we previously reported in the context of ABCA1 or ABCA1-GFP (Tanaka et al. 2003). Login to comment 37 ABCA1 p.Trp590Ser As shown in Fig. S1 in Supporting Information/Supplementary material, the subcellular localization and cell surface expression of the W590S and MM mutants of ABCA1(207HA) (lower panels) were indistinguishable from those of the corresponding mutants of ABCA1-GFP. Login to comment 197 ABCA1 p.Trp590Ser ABCA1 p.Lys939Met ABCA1 p.Lys1952Met DNA construction We constructed plasmids for expressing human wild-type ABCA1, ABCA1(W590S) and ABCA1(K939M,K1952M)(MM) bearing an insertion of the influenza virus hemagglutinin (HA) epitope sequence between residues 207 and 208 (within the first extracellular loop), using the bicistronic expression vector pHaMAIRESneo. Login to comment