ABCMdb

PMID: 18349419

Heredi-Szabo K, Kis E, Molnar E, Gyorfi A, Krajcsi P
Characterization of 5(6)-carboxy-2,'7'-dichlorofluorescein transport by MRP2 and utilization of this substrate as a fluorescent surrogate for LTC4.
J Biomol Screen. 2008 Apr;13(4):295-301. Epub 2008 Mar 18., [PubMed]

Sentences

No. Mutations Sentence Comment

36 ABCC1 p.Gly771Asp Expression of human MRP2 in insect cells Recombinant baculoviruses encoding the wild-type human MRP227 and defective MRP1 mutant G771D (defMRP),28 used as control throughout the studies, were kind gifts from Drs. Balazs Sarkadi (National Medical Center, Budapest, Hungary) and András Váradi (Institute of Enzymology, Budapest, Hungary). Login to comment 37 ABCC1 p.Gly771Asp Sf9 cells were cultured and infected with the recombinant baculovirus stocks as described earlier.27,29 Membrane preparation Membrane vesicle preparations obtained from insect cells expressing ABCC2 (MRP2-Sf9) and defective MRP1 mutant G771D (defMRP-Sf9) were obtained from SOLVO Biotechnology (Szeged, Hungary). Login to comment