ABCMdb

PMID: 16895967

Matsuzono Y, Matsuzaki T, Fujiki Y
Functional domain mapping of peroxin Pex19p: interaction with Pex3p is essential for function and translocation.
J Cell Sci. 2006 Sep 1;119(Pt 17):3539-50. Epub 2006 Aug 8., [PubMed]

Sentences

No. Mutations Sentence Comment

236 ABCD3 p.Asn12Phe To construct PEX19 mutants encoding N-terminally truncated mutants, including ⌬N11 (the same as 12-299), ⌬N23 (24-299), ⌬N39 (40-299) and ⌬N69 (70-299), PCR was done using respective forward primers, N12F/SalI, N24F/SalI, N40F/SalI and N70F/SalI, and the reverse primer C299R/XhoI-NotI. Login to comment 237 ABCD3 p.Asn12Phe ABCD3 p.Asn12Phe For construction of PEX19 variants encoding Pex19p with deletion of amino acid residues 1-11 and 262-299 (named M12-261), M12-255, M40-131 and M40-73, PCR was done using N12F/SalI or N40F/SalI and C261R/XhoI-NotI, C255R/XhoI-NotI, C131R/XhoI-NotI and C73R/XhoI-NotI, Journal of Cell Science 119 (17) respectively. Login to comment 238 ABCD3 p.Asn12Phe For construction of PEX19 variants encoding Pex19p with deletion of amino acid residues 1-11 and 262-299 (named M12-261), M12-255, M40-131 and M40-73, PCR was done using N12F/SalI or N40F/SalI and C261R/XhoI-NotI, C255R/XhoI-NotI, C131R/XhoI-NotI and C73R/XhoI-NotI, Journal of Cell Science 119 (17) respectively. Login to comment 241 ABCD3 p.Asn12Phe For M12-131, M24-131 and M40-131, PCR was done using forward N12F/SalI, N24F/SalI, and N40F/SalI, and a reverse C131R/XhoI-NotI. Login to comment 242 ABCD3 p.Asn12Phe For M12-131, M24-131 and M40-131, PCR was done using forward N12F/SalI, N24F/SalI, and N40F/SalI, and a reverse C131R/XhoI-NotI. Login to comment 244 ABCD3 p.Asn12Phe Myc6-HA2-PEX19 variants encoding 1-131, 1-73 (⌬C226), M12-73, M24-73 and M40-73, was constructed by PCR using Myc6-HA2-PEX19 as template and a set of primers, N2F/SalI, N12F/SalI, N24F/SalI and N40F/SalI, with C131R/XhoI-NotI and C73R/XhoI-NotI. Login to comment 245 ABCD3 p.Asn12Phe Myc6-HA2-PEX19 variants encoding 1-131, 1-73 (èc;C226), M12-73, M24-73 and M40-73, was constructed by PCR using Myc6-HA2-PEX19 as template and a set of primers, N2F/SalI, N12F/SalI, N24F/SalI and N40F/SalI, with C131R/XhoI-NotI and C73R/XhoI-NotI. Login to comment