ABCD3 p.Asn12Phe
| Predicted by SNAP2: | A: D (53%), C: D (75%), D: D (75%), E: D (71%), F: D (91%), G: D (63%), H: D (71%), I: D (75%), K: D (59%), L: D (75%), M: D (75%), P: D (75%), Q: N (53%), R: D (59%), S: N (66%), T: N (66%), V: D (66%), W: D (91%), Y: D (75%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, E: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, P: N, Q: N, R: N, S: N, T: N, V: N, W: D, Y: N, |
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[hide] Functional domain mapping of peroxin Pex19p: inter... J Cell Sci. 2006 Sep 1;119(Pt 17):3539-50. Epub 2006 Aug 8. Matsuzono Y, Matsuzaki T, Fujiki Y
Functional domain mapping of peroxin Pex19p: interaction with Pex3p is essential for function and translocation.
J Cell Sci. 2006 Sep 1;119(Pt 17):3539-50. Epub 2006 Aug 8., [PMID:16895967]
Abstract [show]
The peroxin Pex19p functions in peroxisomal membrane assembly. Here we mapped functional domains of human Pex19p comprising 299 amino acids. Pex19p mutants deleted in the C-terminal CAAx farnesylation motif, the C-terminal 38 amino acid residues and the N-terminal 11 residues, maintained peroxisome-restoring activity in pex19 cells. The sequence 12-261 was essential for re-establishing peroxisome activity. Pex19p was partly localized to peroxisomes but mostly localized in the cytosol. Pex19p interacted with multiple membrane proteins, including the other two membrane biogenesis peroxins, Pex3p and Pex16p, those involved in matrix protein import such as Pex14p, Pex13p, Pex10p, and Pex26p, peroxisome morphogenesis factor Pex11pbeta, and a PMP70 peroxisome-targeting signal region at residues 1-123. In yeast two-hybrid assays, Pex10p and Pex11pbeta interacted only with full-length Pex19p. Of various truncated Pex19p variants active in translocating to peroxisomes, the mutants with the shortest sequence (residues 12-73 and 40-131) were localized to peroxisomes and competent in binding to Pex3p. Furthermore, membrane peroxins were initially discernible in a cytosolic staining pattern in pex19 cells only when co-expressed with Pex19p and were then localized to peroxisomes in a temporally differentiated manner. Pex19p probably functions as a chaperone for membrane proteins and transports them to peroxisomes by anchoring to Pex3p using residues 12-73 and 40-131.
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No. Sentence Comment
236 To construct PEX19 mutants encoding N-terminally truncated mutants, including ⌬N11 (the same as 12-299), ⌬N23 (24-299), ⌬N39 (40-299) and ⌬N69 (70-299), PCR was done using respective forward primers, N12F/SalI, N24F/SalI, N40F/SalI and N70F/SalI, and the reverse primer C299R/XhoI-NotI.
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ABCD3 p.Asn12Phe 16895967:236:228
status: NEW237 For construction of PEX19 variants encoding Pex19p with deletion of amino acid residues 1-11 and 262-299 (named M12-261), M12-255, M40-131 and M40-73, PCR was done using N12F/SalI or N40F/SalI and C261R/XhoI-NotI, C255R/XhoI-NotI, C131R/XhoI-NotI and C73R/XhoI-NotI, Journal of Cell Science 119 (17) respectively.
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ABCD3 p.Asn12Phe 16895967:237:170
status: NEWX
ABCD3 p.Asn12Phe 16895967:237:224
status: NEW241 For M12-131, M24-131 and M40-131, PCR was done using forward N12F/SalI, N24F/SalI, and N40F/SalI, and a reverse C131R/XhoI-NotI.
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ABCD3 p.Asn12Phe 16895967:241:61
status: NEW244 Myc6-HA2-PEX19 variants encoding 1-131, 1-73 (⌬C226), M12-73, M24-73 and M40-73, was constructed by PCR using Myc6-HA2-PEX19 as template and a set of primers, N2F/SalI, N12F/SalI, N24F/SalI and N40F/SalI, with C131R/XhoI-NotI and C73R/XhoI-NotI.
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ABCD3 p.Asn12Phe 16895967:244:176
status: NEW238 For construction of PEX19 variants encoding Pex19p with deletion of amino acid residues 1-11 and 262-299 (named M12-261), M12-255, M40-131 and M40-73, PCR was done using N12F/SalI or N40F/SalI and C261R/XhoI-NotI, C255R/XhoI-NotI, C131R/XhoI-NotI and C73R/XhoI-NotI, Journal of Cell Science 119 (17) respectively.
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ABCD3 p.Asn12Phe 16895967:238:170
status: NEW242 For M12-131, M24-131 and M40-131, PCR was done using forward N12F/SalI, N24F/SalI, and N40F/SalI, and a reverse C131R/XhoI-NotI.
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ABCD3 p.Asn12Phe 16895967:242:61
status: NEW245 Myc6-HA2-PEX19 variants encoding 1-131, 1-73 (èc;C226), M12-73, M24-73 and M40-73, was constructed by PCR using Myc6-HA2-PEX19 as template and a set of primers, N2F/SalI, N12F/SalI, N24F/SalI and N40F/SalI, with C131R/XhoI-NotI and C73R/XhoI-NotI.
X
ABCD3 p.Asn12Phe 16895967:245:175
status: NEW