ABCMdb

PMID: 16149910

Liu X, Luo M, Zhang LN, Yan Z, Zak R, Ding W, Mansfield SG, Mitchell LG, Engelhardt JF
Spliceosome-mediated RNA trans-splicing with recombinant adeno-associated virus partially restores cystic fibrosis transmembrane conductance regulator function to polarized human cystic fibrosis airway epithelial cells.
Hum Gene Ther. 2005 Sep;16(9):1116-23., [PubMed]

Sentences

No. Mutations Sentence Comment

38 ABCC7 p.Asn1303Lys ABCC7 p.Arg560Thr Generation of polarized human airway epithelia and rAAV infection Freshly isolated human airway epithelial cells from either non-CF bronchial airways, or CF bronchial airways with ⌬F508/⌬F508, ⌬F508/3659⌬C, ⌬F508/R560T, and ⌬F508/ N1303K mutations, were seeded into collagen-coated 0.6-cm2 Millicell culture inserts (Millipore, Bedford, MA) and grown at the air-liquid interface in 24-well tissue culture plates containing 0.5 ml of medium in the basolateral compartment as previously described (Karp et al., 2002). Login to comment 69 ABCC7 p.Asn1303Lys ABCC7 p.Arg560Thr The polarized human CF airway epithelial cells used in this study included several genotypes, all of which contained at least one ⌬F508 allele (⌬F508/⌬F508, ⌬F508/3659⌬C, ⌬F508/R560T, and ⌬F508/N1303K). Login to comment