ABCMdb

PMID: 15923638

Farinha CM, Amaral MD
Most F508del-CFTR is targeted to degradation at an early folding checkpoint and independently of calnexin.
Mol Cell Biol. 2005 Jun;25(12):5242-52., [PubMed]

Sentences

No. Mutations Sentence Comment

208 ABCC7 p.Asn894Ala ABCC7 p.Asn894Gln ABCC7 p.Asn900Gln ABCC7 p.Asn900Ala As the N-glyconjugate is attached to asparagine residues 894 and 900 of CFTR, we replaced both these residues by either glutamine (N894Q and N900Q) or alanine (N894A and N900A) residues and studied the CFTR turnover of the resulting proteins on wt and F508del backgrounds when stably expressed in BHK cells as above. Login to comment 219 ABCC7 p.Asn894Ala ABCC7 p.Asn894Ala ABCC7 p.Asn894Gln ABCC7 p.Asn894Gln ABCC7 p.Asn900Gln ABCC7 p.Asn900Gln ABCC7 p.Asn900Ala ABCC7 p.Asn900Ala BHK cells stably expressing wt CFTR (A, lanes 1 to 5), N894A-N900A (WAA) (A, lanes 6 to 10), N894Q-N900Q (WQQ) (A, lanes 11 to 15), F508del-CFTR (B, lanes 1 to 5), F508del-N894A-N900A (FAA) (B, lanes 6 to 10), or F508del-N894Q-N900Q CFTR (FQQ) (B, lanes 11 to 15) were pulse-labeled and chased as before (Fig. 2) for 0 h (lanes 1, 6, and 11), 0.5 h (lanes 2, 7, and 12), 1 h (lanes 3, 8, and 13), 2 h (lanes 4, 9, and 14), and 3 h (lanes 5, 10, and 15). Login to comment 284 ABCC7 p.Asn894Ala ABCC7 p.Asn894Gln ABCC7 p.Asn900Gln ABCC7 p.Asn900Ala Asparagine residues at positions 894 and 900 were replaced by either two alanines (N894A and N900A) or two glutamines (N894Q and N900Q). Login to comment