PMID: 15761017

Ungureanu D, Vanhatupa S, Gronholm J, Palvimo JJ, Silvennoinen O
SUMO-1 conjugation selectively modulates STAT1-mediated gene responses.
Blood. 2005 Jul 1;106(1):224-6. Epub 2005 Mar 10., [PubMed]
Sentences
No. Mutations Sentence Comment
10 ABCB2 p.Glu3Ala
X
ABCB2 p.Glu3Ala 15761017:10:158
status: NEW
view ABCB2 p.Glu3Ala details
Plasmids The SUMO-1, STAT1-wild-type-HA (STAT1-WT-HA), and STAT1-KR-HA plasmids have been previously described.3 STAT1-I702R-HA (Ile3Arg) and STAT1-E705A-HA (Glu3Ala) were constructed from STAT1-WT-HA using polymerase chain reaction (PCR) mutagenesis with the following primers: 5b18;-GGAACTGGATAT- AGGAAGACTGAGTTGATTTCTGTGTCTGAA-3b18; and 5b18;-TTCAGA- CACAGAAATCAACTCAGTCTTCCTATATCCAGTTCC-3b18;; 5b18;-GGA- ACTGGATATATCAAGACTGCGTTGATTTCTGTGTCTGAA-3b18; and 5b18;-TTCAGACACAGAAATCAACGCAGTCTTGATATATCCAGTTCC-3b18;. Login to comment
11 ABCB2 p.Glu3Ala
X
ABCB2 p.Glu3Ala 15761017:11:158
status: NEW
view ABCB2 p.Glu3Ala details
Plasmids The SUMO-1, STAT1-wild-type-HA (STAT1-WT-HA), and STAT1-KR-HA plasmids have been previously described.3 STAT1-I702R-HA (Ile3Arg) and STAT1-E705A-HA (Glu3Ala) were constructed from STAT1-WT-HA using polymerase chain reaction (PCR) mutagenesis with the following primers: 5Ј-GGAACTGGATAT- AGGAAGACTGAGTTGATTTCTGTGTCTGAA-3Ј and 5Ј-TTCAGA- CACAGAAATCAACTCAGTCTTCCTATATCCAGTTCC-3Ј; 5Ј-GGA- ACTGGATATATCAAGACTGCGTTGATTTCTGTGTCTGAA-3Ј and 5Ј-TTCAGACACAGAAATCAACGCAGTCTTGATATATCCAGTTCC-3Ј. Login to comment