ABCB2 p.Glu3Ala
| Predicted by SNAP2: | A: N (72%), C: N (72%), D: N (82%), F: N (57%), G: N (61%), H: N (72%), I: N (61%), K: N (66%), L: N (57%), M: N (72%), N: N (78%), P: D (53%), Q: N (82%), R: N (57%), S: N (78%), T: N (78%), V: N (66%), W: N (57%), Y: N (61%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, N: N, P: N, Q: N, R: N, S: N, T: N, V: N, W: N, Y: N, |
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[hide] SUMO-1 conjugation selectively modulates STAT1-med... Blood. 2005 Jul 1;106(1):224-6. Epub 2005 Mar 10. Ungureanu D, Vanhatupa S, Gronholm J, Palvimo JJ, Silvennoinen O
SUMO-1 conjugation selectively modulates STAT1-mediated gene responses.
Blood. 2005 Jul 1;106(1):224-6. Epub 2005 Mar 10., [PMID:15761017]
Abstract [show]
Signal transducers and activators of transcription 1 (STAT1) is a critical mediator of interferon (IFN)-induced gene responses. Recently, STAT1 was found to become modified by small ubiquitin-like modifier 1 (SUMO-1) conjugation at Lys703 through the SUMO E3 ligase function of protein inhibitors of activated STAT (PIAS) proteins. However, the physiologic function of sumoylation in STAT1 is still unclear. Here, we show that mutations in the SUMO attachment site in STAT1 result in increased transcriptional activity in a fashion that is selective among IFN-gamma target genes. The sumoylation-defective STAT1 mutant displayed increased induction of guanylate-binding protein 1 (GBP1) and transporters associated with antigen presentation 1 (TAP1) transcription but not interferon regulatory factor 1 (IRF1) transcription. Moreover, the sumoylation-defective mutant STAT1-KR showed a prolonged DNA-binding activity and nuclear localization in response to IFN-gamma stimulation. These results suggest that sumoylation has a defined negative regulatory effect on selective STAT1-mediated transcription responses.
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11 Plasmids The SUMO-1, STAT1-wild-type-HA (STAT1-WT-HA), and STAT1-KR-HA plasmids have been previously described.3 STAT1-I702R-HA (Ile3Arg) and STAT1-E705A-HA (Glu3Ala) were constructed from STAT1-WT-HA using polymerase chain reaction (PCR) mutagenesis with the following primers: 5Ј-GGAACTGGATAT- AGGAAGACTGAGTTGATTTCTGTGTCTGAA-3Ј and 5Ј-TTCAGA- CACAGAAATCAACTCAGTCTTCCTATATCCAGTTCC-3Ј; 5Ј-GGA- ACTGGATATATCAAGACTGCGTTGATTTCTGTGTCTGAA-3Ј and 5Ј-TTCAGACACAGAAATCAACGCAGTCTTGATATATCCAGTTCC-3Ј.
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ABCB2 p.Glu3Ala 15761017:11:158
status: NEW10 Plasmids The SUMO-1, STAT1-wild-type-HA (STAT1-WT-HA), and STAT1-KR-HA plasmids have been previously described.3 STAT1-I702R-HA (Ile3Arg) and STAT1-E705A-HA (Glu3Ala) were constructed from STAT1-WT-HA using polymerase chain reaction (PCR) mutagenesis with the following primers: 5b18;-GGAACTGGATAT- AGGAAGACTGAGTTGATTTCTGTGTCTGAA-3b18; and 5b18;-TTCAGA- CACAGAAATCAACTCAGTCTTCCTATATCCAGTTCC-3b18;; 5b18;-GGA- ACTGGATATATCAAGACTGCGTTGATTTCTGTGTCTGAA-3b18; and 5b18;-TTCAGACACAGAAATCAACGCAGTCTTGATATATCCAGTTCC-3b18;.
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ABCB2 p.Glu3Ala 15761017:10:158
status: NEW