ABCMdb

PMID: 15290302

Ai T, Bompadre SG, Sohma Y, Wang X, Li M, Hwang TC
Direct effects of 9-anthracene compounds on cystic fibrosis transmembrane conductance regulator gating.
Pflugers Arch. 2004 Oct;449(1):88-95., [PubMed]

Sentences

No. Mutations Sentence Comment

32 ABCC7 p.Lys1250Ala Materials and methods Cell culture NIH3T3 cells stably expressing wild-type CFTR (NIH3T3/CFTR) or K1250A-CFTR mutant channels were grown as previously described [32] at 37°C and 5% CO2 in DMEM supplemented with 10% FBS. Login to comment 78 ABCC7 p.Lys1250Ala Since K1250A-CFTR channels Fig. 1A-D Effects of anthracene-9-carboxylic acid (9-AC) on whole-cell cystic fibrosis transmembrane conductance regulator (CFTR) currents. Login to comment 82 ABCC7 p.Lys1250Ala Net potentiation effects in wild-type CFTR were calculated by correcting for the inhibitory effects obtained from recordings of 9-AC on K1250A-CFTR currents. Login to comment 83 ABCC7 p.Lys1250Ala ycomp=yraw/(1-x/100), where ycomp, is the compensated value; yraw, the measured fold increase; x, the percentage inhibition of whole-cell K1250A-CFTR currents with 1 mM 9-AC at a pipette potential (Vp) of +100 mV or -100 mV. Login to comment 85 ABCC7 p.Lys1250Ala C A whole-cell K1250A-CFTR current trace. Login to comment 86 ABCC7 p.Lys1250Ala I-V relationships show net K1250A-CFTR currents in the presence or absence of 9-AC. Login to comment 87 ABCC7 p.Lys1250Ala D Dose-response relationships of the inhibitory effect on K1250A-CFTR currents at three different voltages. Login to comment 91 ABCC7 p.Lys1250Ala Figure 1C shows the whole-cell current trace from an NIH3T3 cell stably expressing K1250A-CFTR. Login to comment 93 ABCC7 p.Lys1250Ala Figure 1D shows the dose-response relationships of this inhibitory effect of 9-AC on K1250A-CFTR at three different membrane potentials (-60, -80, and -100 mV). Login to comment