ABCMdb

PMID: 15208386

Goodman CD, Casati P, Walbot V
A multidrug resistance-associated protein involved in anthocyanin transport in Zea mays.
Plant Cell. 2004 Jul;16(7):1812-26. Epub 2004 Jun 18., [PubMed]

Sentences

No. Mutations Sentence Comment

300 ABCC5 p.Asp143Arg ABCC3 p.Asp167Arg Tissue-Specific Probes for RNA Gel Blots Gene Probe Location Probe Construction ZmMrp1 39 coding/UTR 936-bp Product from 39RACE clone digested with NcoI and NotI ZmMrp2 39 UTR 368-bp PCR product: D156F 59-AGCCGCAATGGAATGGTTGT- 39 and D157R 59-CGTCAGG- CCTTTTAGAACAA-39 ZmMrp3 39 UTR 405-bp PCR product: D63F 59-GTGACGGGAAAGTAGTGGAGTA-39 and D64R 59-CTGCCG- CACAAGCATTTCTGT-39 ZmMrp3 59 UTR/coding 212-bp PCR product: D78F 59-CATCGATCCGCGCTTTGCTTGATGGGATAA-39 and D110R 59-GCAGCTGGAGGAGTCGAGTATGTC-39 ZmMrp4 39 UTR 226-bp PCR product: DSP36F 59-ATATCTAGAAGCCACCTC-39 and D143R 59-TGGAAGGG- AAGCAATGAC-39 ZmMrp5 39 coding/UTR 604-bp Product from EST clone digested with EcoRV and HindIII ZmMrp6 39 coding/UTR 736-bp Product from EST clone digested with HindIII ZmMrp7 39 UTR 293-bp PCR product: D58F 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 and D57R 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 ZmMrp8 39 UTR 329-bp PCR product: D150F 59-TGAAGCGGAGCAAGATCCTC-39 and D151R 59-CGTGGA- GCCAAAGCAACCTT-39 ZmMrp9 39 UTR 356-bp PCR product: D166F 59-CTCAGATCTCGCTTTGGCATA-39 and D167R 59-ATCTGG- GTCTGCTTCAGTATG-39 ZmMrp10 39 UTR 402-bp PCR product: D168F 59-CCCAGGTCAGCGTCAACTTGT-39 and D169R 59-CACAC- TTCCTAGGGCGTTTGT-39 ZmAbcf1 39 coding/UTR 450-bp Product from EST clone cut with SstI and NotI involved in anthocyanin sequestration in these tissues. Login to comment 301 ABCC5 p.Asp143Arg ABCC3 p.Asp167Arg Tissue-Specific Probes for RNA Gel Blots Gene Probe Location Probe Construction ZmMrp1 39 coding/UTR 936-bp Product from 39RACE clone digested with NcoI and NotI ZmMrp2 39 UTR 368-bp PCR product: D156F 59-AGCCGCAATGGAATGGTTGT- 39 and D157R 59-CGTCAGG- CCTTTTAGAACAA-39 ZmMrp3 39 UTR 405-bp PCR product: D63F 59-GTGACGGGAAAGTAGTGGAGTA-39 and D64R 59-CTGCCG- CACAAGCATTTCTGT-39 ZmMrp3 59 UTR/coding 212-bp PCR product: D78F 59-CATCGATCCGCGCTTTGCTTGATGGGATAA-39 and D110R 59-GCAGCTGGAGGAGTCGAGTATGTC-39 ZmMrp4 39 UTR 226-bp PCR product: DSP36F 59-ATATCTAGAAGCCACCTC-39 and D143R 59-TGGAAGGG- AAGCAATGAC-39 ZmMrp5 39 coding/UTR 604-bp Product from EST clone digested with EcoRV and HindIII ZmMrp6 39 coding/UTR 736-bp Product from EST clone digested with HindIII ZmMrp7 39 UTR 293-bp PCR product: D58F 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 and D57R 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 ZmMrp8 39 UTR 329-bp PCR product: D150F 59-TGAAGCGGAGCAAGATCCTC-39 and D151R 59-CGTGGA- GCCAAAGCAACCTT-39 ZmMrp9 39 UTR 356-bp PCR product: D166F 59-CTCAGATCTCGCTTTGGCATA-39 and D167R 59-ATCTGG- GTCTGCTTCAGTATG-39 ZmMrp10 39 UTR 402-bp PCR product: D168F 59-CCCAGGTCAGCGTCAACTTGT-39 and D169R 59-CACAC- TTCCTAGGGCGTTTGT-39 ZmAbcf1 39 coding/UTR 450-bp Product from EST clone cut with SstI and NotI involved in anthocyanin sequestration in these tissues. Login to comment 355 ABCC1 p.Asp88Phe For ZmMrp3: D109F, 59-CCA- TCGATATCCGCACGCGCCTTTAGCTACTA-39, and D95R, 59-TACC- GCGGATTAGATGTGTATGACCAGTACTC-39; for ZmABCF1I: D88F, 59-GCGGATCCACCCAACCCAACCAAGAAACAGAGG-39, and D89R, 59-GGAATTCATAGCATACGGCACAGCACGCAGAGT-39. Login to comment 356 ABCC1 p.Asp88Phe For ZmMrp3: D109F, 59-CCA- TCGATATCCGCACGCGCCTTTAGCTACTA-39, and D95R, 59-TACC- GCGGATTAGATGTGTATGACCAGTACTC-39; for ZmABCF1I: D88F, 59-GCGGATCCACCCAACCCAACCAAGAAACAGAGG-39, and D89R, 59-GGAATTCATAGCATACGGCACAGCACGCAGAGT-39. Login to comment