ABCC3 p.Asp167Arg
| Predicted by SNAP2: | A: D (53%), C: D (71%), E: N (53%), F: D (71%), G: D (75%), H: D (80%), I: D (71%), K: D (66%), L: D (75%), M: D (71%), N: N (57%), P: D (85%), Q: D (53%), R: D (80%), S: N (61%), T: D (71%), V: D (71%), W: D (75%), Y: D (75%), |
| Predicted by PROVEAN: | A: D, C: D, E: N, F: D, G: D, H: D, I: D, K: D, L: D, M: D, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D, |
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[hide] A multidrug resistance-associated protein involved... Plant Cell. 2004 Jul;16(7):1812-26. Epub 2004 Jun 18. Goodman CD, Casati P, Walbot V
A multidrug resistance-associated protein involved in anthocyanin transport in Zea mays.
Plant Cell. 2004 Jul;16(7):1812-26. Epub 2004 Jun 18., [PMID:15208386]
Abstract [show]
Anthocyanin biosynthesis is one of the most thoroughly studied enzymatic pathways in biology, but little is known about the molecular mechanisms of its final stage: the transport of the anthocyanin pigment into the vacuole. We have identified a multidrug resistance-associated protein (MRP), ZmMrp3, that is required for this transport process in maize (Zea mays). ZmMrp3 expression is controlled by the regulators of anthocyanin biosynthesis and mirrors the expression of other anthocyanin structural genes. Localization of ZmMRP3 in vivo shows its presence in the tonoplast, the site at which anthocyanin transport occurs. Mutants generated using antisense constructs have a distinct pigmentation phenotype in the adult plant that results from a mislocalization of the pigment as well as significant reduction in anthocyanin content, with no alteration in the anthocyanin species produced. Surprisingly, mutant plants did not show a phenotype in the aleurone. This appears to reflect the presence of a second, highly homologous gene, ZmMrp4, that is also coregulated with the anthocyanin pathway but is expressed exclusively in aleurone tissue. This description of a plant MRP with a role in the transport of a known endogenous substrate provides a new model system for examining the biological and biochemical mechanisms involved in the MRP-mediated transport of plant secondary metabolites.
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No. Sentence Comment
301 Tissue-Specific Probes for RNA Gel Blots Gene Probe Location Probe Construction ZmMrp1 39 coding/UTR 936-bp Product from 39RACE clone digested with NcoI and NotI ZmMrp2 39 UTR 368-bp PCR product: D156F 59-AGCCGCAATGGAATGGTTGT- 39 and D157R 59-CGTCAGG- CCTTTTAGAACAA-39 ZmMrp3 39 UTR 405-bp PCR product: D63F 59-GTGACGGGAAAGTAGTGGAGTA-39 and D64R 59-CTGCCG- CACAAGCATTTCTGT-39 ZmMrp3 59 UTR/coding 212-bp PCR product: D78F 59-CATCGATCCGCGCTTTGCTTGATGGGATAA-39 and D110R 59-GCAGCTGGAGGAGTCGAGTATGTC-39 ZmMrp4 39 UTR 226-bp PCR product: DSP36F 59-ATATCTAGAAGCCACCTC-39 and D143R 59-TGGAAGGG- AAGCAATGAC-39 ZmMrp5 39 coding/UTR 604-bp Product from EST clone digested with EcoRV and HindIII ZmMrp6 39 coding/UTR 736-bp Product from EST clone digested with HindIII ZmMrp7 39 UTR 293-bp PCR product: D58F 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 and D57R 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 ZmMrp8 39 UTR 329-bp PCR product: D150F 59-TGAAGCGGAGCAAGATCCTC-39 and D151R 59-CGTGGA- GCCAAAGCAACCTT-39 ZmMrp9 39 UTR 356-bp PCR product: D166F 59-CTCAGATCTCGCTTTGGCATA-39 and D167R 59-ATCTGG- GTCTGCTTCAGTATG-39 ZmMrp10 39 UTR 402-bp PCR product: D168F 59-CCCAGGTCAGCGTCAACTTGT-39 and D169R 59-CACAC- TTCCTAGGGCGTTTGT-39 ZmAbcf1 39 coding/UTR 450-bp Product from EST clone cut with SstI and NotI involved in anthocyanin sequestration in these tissues.
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ABCC3 p.Asp167Arg 15208386:301:1063
status: NEW300 Tissue-Specific Probes for RNA Gel Blots Gene Probe Location Probe Construction ZmMrp1 39 coding/UTR 936-bp Product from 39RACE clone digested with NcoI and NotI ZmMrp2 39 UTR 368-bp PCR product: D156F 59-AGCCGCAATGGAATGGTTGT- 39 and D157R 59-CGTCAGG- CCTTTTAGAACAA-39 ZmMrp3 39 UTR 405-bp PCR product: D63F 59-GTGACGGGAAAGTAGTGGAGTA-39 and D64R 59-CTGCCG- CACAAGCATTTCTGT-39 ZmMrp3 59 UTR/coding 212-bp PCR product: D78F 59-CATCGATCCGCGCTTTGCTTGATGGGATAA-39 and D110R 59-GCAGCTGGAGGAGTCGAGTATGTC-39 ZmMrp4 39 UTR 226-bp PCR product: DSP36F 59-ATATCTAGAAGCCACCTC-39 and D143R 59-TGGAAGGG- AAGCAATGAC-39 ZmMrp5 39 coding/UTR 604-bp Product from EST clone digested with EcoRV and HindIII ZmMrp6 39 coding/UTR 736-bp Product from EST clone digested with HindIII ZmMrp7 39 UTR 293-bp PCR product: D58F 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 and D57R 59-AGTGGATCCGCACGAGTTATTCCCTTGGTTCT-39 ZmMrp8 39 UTR 329-bp PCR product: D150F 59-TGAAGCGGAGCAAGATCCTC-39 and D151R 59-CGTGGA- GCCAAAGCAACCTT-39 ZmMrp9 39 UTR 356-bp PCR product: D166F 59-CTCAGATCTCGCTTTGGCATA-39 and D167R 59-ATCTGG- GTCTGCTTCAGTATG-39 ZmMrp10 39 UTR 402-bp PCR product: D168F 59-CCCAGGTCAGCGTCAACTTGT-39 and D169R 59-CACAC- TTCCTAGGGCGTTTGT-39 ZmAbcf1 39 coding/UTR 450-bp Product from EST clone cut with SstI and NotI involved in anthocyanin sequestration in these tissues.
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ABCC3 p.Asp167Arg 15208386:300:1063
status: NEW