ABCMdb

PMID: 14533738

Landgraf P, Mayerhofer PU, Polanetz R, Roscher AA, Holzinger A
Targeting of the human adrenoleukodystrophy protein to the peroxisomal membrane by an internal region containing a highly conserved motif.
Eur J Cell Biol. 2003 Aug;82(8):401-10., [PubMed]

Sentences

No. Mutations Sentence Comment

7 ABCD1 p.Leu78Phe The single amino acid substitution L78F resulted in a significant reduction of targeting efficiency. Login to comment 127 ABCD1 p.Leu78Phe ABCD1 p.Leu78Phe ABCD1 p.Arg74Trp ABCD1 p.Arg74Trp ABCD1 p.Pro84Ala ABCD1 p.Pro84Ala ABCD1 p.Phe71Ser ABCD1 p.Phe71Ser ABCD1 p.Phe71Val ABCD1 p.Phe71Val ABCD1 p.Leu81Val ABCD1 p.Leu81Val ABCD1 p.Arg74Gly ABCD1 p.Arg74Gly Construct name n Strictly peroxisomal targeting (%) 1 ± 281 ALDP-GFP1 361 313 (86.7) * 1 ± 164 ALDP-GFP1 331 277 (83.7) * 1 ± 110 ALDP-GFP1 132 108 (81.8) * 282 ± 745 ALDP-GFP 218 0 (0) * 67 ± 164 ALDP-GFP 176 90 (51.1) 67 ± 134 ALDP-GFP 275 0 (0) * 78 ± 164 ALDP-GFP2 247 2 (0.8) * 87 ± 164 ALDP-GFP2 206 1 (0.5) * 67 ± 164 ALDP(F71S)-GFP3 363 143 (39.4) 67 ± 164 ALDP(F71V)-GFP3 203 90 (44.3) 67 ±; 164 ALDP(R74W)-GFP3 218 89 (40.8) 67 1; 164 ALDP(R74G)-GFP3 277 123 (44.4) 67 ± 164 ALDP(L78F)-GFP3 315 103 (32.7) * 67 ± 164 ALDP(L81V)-GFP3 363 199 (54.8) 67 ± 164 ALDP(P84A)-GFP3 413 196 (47.5) 67 ± 164 ALDP-delLLR-GFP4 224 0 (0) * Total number of cells (n) analysed that were transfected with various ALDP-GFP fusion constructs. Login to comment 149 ABCD1 p.Leu78Phe ABCD1 p.Leu78Phe The percentage of 67 ± 164 ALDP(L78F)-GFP-transfected cells exhibiting a strictly peroxisomal pattern significantly (p < 0.01) differed as compared to the wild-type 67 ± 164 ALDP-GFP (Table II) suggesting a decrease in targeting efficiency. Login to comment 179 ABCD1 p.Leu78Phe ABCD1 p.Leu78Phe Among several single-amino-acid substitutions of conserved residues only the 67 ± 164 ALDP(L78F)-GFP construct significantly reduced targeting efficiency. Login to comment