ABCMdb

PMID: 11739236

Taylor AM, Storm J, Soceneantu L, Linton KJ, Gabriel M, Martin C, Woodhouse J, Blott E, Higgins CF, Callaghan R
Detailed characterization of cysteine-less P-glycoprotein reveals subtle pharmacological differences in function from wild-type protein.
Br J Pharmacol. 2001 Dec;134(8):1609-18., [PubMed]

Sentences

No. Mutations Sentence Comment

83 ABCB1 p.Cys137Ser ABCB1 p.Cys1227Ser ABCB1 p.Cys1125Ser ABCB1 p.Cys431Ser ABCB1 p.Cys717Ser ABCB1 p.Cys956Ser ABCB1 p.Cys1074Ser ATP hydrolysis was stopped by addition of 40 ml of a 12% (w v71 ) SDS solution and released inorganic Table 1 Oligonucleotide sequences used to generate cDNA for P-gp devoid of cysteine residues Oligonucleotide Mutation Sequence (5'-3') 1 C137S GTTTCATTTTGGaGCCTGGCAGCTG 2 C431S GAAACAGTGGCTcTGGGAAGAGCAC 3 C717S TTGGTGTATTTTcTGCCATTATAAA 4 C956S TTTCCTATGCTGGATcTTTCCGGTTTGGAGC 5 C1074S GCAGCAGTGGCTcTGGGAAGAGCAC 6 C1125S-PstI+Xhol ATCCTGTTTGACTcgAGCATTGCTGAGA 7 C1227S GAAGGCCGCACCaGCATTGTGATTG 8 6xHis+XbaI+NotI AGGCTGGAACAAAGCGCCAGcaccatcaccatcaccactgatctagagcggccgc ATTTGTTTAGATATGACATTT 9 _BstEII (nt 671) GCAAATGCAGGtAAccTAGAAGATCTGATG 10 +PstI (nt 844) GGAGCCTGGCtGCaGGAAGACAAATA 11 +SacII (nt 1054) GTAGGATTTACcCGcGGTTGGAAGCTAACC 12 +SfiI (nt 1304) GGGATAAAGAAgGCcATTACgGCCAATATTTC 13 +AflII (nt 1387) GGGACCACCTTGGTCtTaagcGGGGAATATTCT 14 +SphI (nt 2812) CATGGTTTTCCGAagCATGCTCAGACAGG 15 7NdeI (nt 3177) AAGTTTGAACAcATGTATGCTCAG 16 +SpeI (nt 3349) GAGGATGTTCTacTAGTATTTTCAGC 17 +EagI (nt 4168) GTGGTGTTTCAGAAcGGCcGAGTCAAGGAGCATGGC phosphate was determined colorimetrically, as previously described (Chiet et al., 1988). Login to comment