ABCMdb

PMID: 11507101

Qian YM, Qiu W, Gao M, Westlake CJ, Cole SP, Deeley RG
Characterization of binding of leukotriene C4 by human multidrug resistance protein 1: evidence of differential interactions with NH2- and COOH-proximal halves of the protein.
J Biol Chem. 2001 Oct 19;276(42):38636-44. Epub 2001 Aug 15., 2001-10-19 [PubMed]

Sentences

No. Mutations Sentence Comment

149 ABCC1 p.Lys1333Met ABCC1 p.Lys684Met Consistent with the suggestion that the two NBDs of MRP1 fulfill different functional roles, our previous studies demonstrated that Walker A mutations, K684M and K1333M, in NBD1 and NBD2, respectively, had different effects on the ability of the protein to transport LTC4 (25). Login to comment 151 ABCC1 p.Lys1333Met ABCC1 p.Lys1333Met ABCC1 p.Lys684Met ABCC1 p.Lys684Met As shown in Fig. 8C, either the double mutation K684M/K1333M or the single mutation K1333M abolished the ability of vanadate and ATP to inhibit LTC4 binding, whereas mutation K684M did not. Login to comment 235 ABCC1 p.Lys1333Met ABCC1 p.Lys684Met C, effect of vanadate trapping on [3 H]LTC4 labeling of co-expressed expressed mutant forms of MRP11-932 and MRP1932-1531 in which NBD1 or NBD2 had been in activated by mutation of essential Walker A mutations (K684M in NBD1 and K1333M in NBD). Login to comment