PMID: 10562541

Tector M, Hartl FU
An unstable transmembrane segment in the cystic fibrosis transmembrane conductance regulator.
EMBO J. 1999 Nov 15;18(22):6290-8., 1999-11-15 [PubMed]
Sentences
No. Mutations Sentence Comment
80 ABCC7 p.Arg334Leu
X
ABCC7 p.Arg334Leu 10562541:80:104
status: NEW
view ABCC7 p.Arg334Leu details
ABCC7 p.Lys335Ile
X
ABCC7 p.Lys335Ile 10562541:80:114
status: NEW
view ABCC7 p.Lys335Ile details
 (C) Translation and translocation into microsomes of constructs containing wild-type or doubly mutated, R334L and K335I, Tm6 were analysed by immunoprecipitation with anti-myc antibody (lanes 1, 2, 5 and 6) or a non-relevant control antibody (NRA) (lanes 3 and 7) from TX-100 extracts of the isolated microsomes. Login to comment 95 ABCC7 p.Arg334Leu
X
ABCC7 p.Arg334Leu 10562541:95:0
status: NEW
view ABCC7 p.Arg334Leu details
ABCC7 p.Lys335Ile
X
ABCC7 p.Lys335Ile 10562541:95:40
status: NEW
view ABCC7 p.Lys335Ile details
 Arginine 334 was changed to leucine and lysine 335 to isoleucine, generating construct Tm5-6(RK-LI)-Inv (Figure 3A). Login to comment 203 ABCC7 p.Arg334Leu
X
ABCC7 p.Arg334Leu 10562541:203:62
status: NEW
view ABCC7 p.Arg334Leu details
ABCC7 p.Lys335Ile
X
ABCC7 p.Lys335Ile 10562541:203:99
status: NEW
view ABCC7 p.Lys335Ile details
 These mutations enabled the production of proteins containing leucine in place of arginine 334 and isoleucine in place of lysine 335 in Tm6 of CFTR. Login to comment