ABCMdb

ABCC7 p.Lys615Ala

Predicted by SNAP2:	A: D (71%), C: D (66%), D: D (91%), E: D (80%), F: D (80%), G: D (80%), H: D (59%), I: D (75%), L: N (57%), M: D (66%), N: D (71%), P: D (85%), Q: N (87%), R: N (82%), S: D (66%), T: D (71%), V: D (75%), W: D (85%), Y: D (75%),
Predicted by PROVEAN:	A: D, C: D, D: D, E: N, F: D, G: D, H: N, I: D, L: D, M: D, N: N, P: D, Q: N, R: N, S: N, T: D, V: D, W: D, Y: N,

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Publications
[hide] Role of CFTR's PDZ1-binding domain, NBF1 and Cl(-)... Biochim Biophys Acta. 2001 Nov 1;1515(1):64-71. Boucherot A, Schreiber R, Kunzelmann K
Role of CFTR's PDZ1-binding domain, NBF1 and Cl(-) conductance in inhibition of epithelial Na(+) channels in Xenopus oocytes.
Biochim Biophys Acta. 2001 Nov 1;1515(1):64-71., [PMID:11597353]

Abstract [show]
The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits epithelial Na(+) channels (ENaC). Evidence has accumulated that both Cl(-) transport through CFTR Cl(-) channels and the first nucleotide binding domain (NBF1) of CFTR are crucial for inhibition of ENaC. A PDZ binding domain (PDZ-BD) at the C-terminal end links CFTR to scaffolding and cytoskeletal proteins, which have been suggested to play an important role in activation of CFTR and eventually inhibition of ENaC. We eliminated the PDZ-BD of CFTR and coexpressed Na(+)/H(+)-exchange regulator factors together with CFTR and ENaC. The results do not support a role of PDZ-BD in inhibition of ENaC by CFTR. However, inhibition of ENaC was closely linked to Cl(-) currents generated by CFTR and was observed in the presence of Cl(-), I(-) or Br(-) but not gluconate. Therefore, functional NBF1 and Cl(-) transport are required for inhibition of ENaC in Xenopus oocytes, while the PDZ-BD is not essential.

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Sentences [show]
No. Sentence Comment
38 Using similar PCR techniques, the NBF1 mutants of human CFTR vF508, G551D, S466L, K464A, D572N, KH483/484AA, R487Q, R516A, KR598/600GA, KK611/612AA and K615A were in vitro synthesized (Quickchange, Stratagene). Login to comment
116 The CFTR mutants K646A, R487Q, G551D, D572N and K615A did not generate signi'cant CFTR Cl3 conductances. Login to comment
172 Furthermore, NBF1 sequences which could potentially serve as activator sequences for G proteins were mutated (KR598/600GA, KK611/ 612AA, K615A). Login to comment