ABCB5 p.Ala375Pro
| Predicted by SNAP2: | C: N (78%), D: N (93%), E: N (97%), F: N (61%), G: N (93%), H: N (93%), I: N (97%), K: N (97%), L: N (93%), M: N (87%), N: N (93%), P: N (93%), Q: N (93%), R: N (93%), S: N (93%), T: N (97%), V: N (93%), W: N (57%), Y: N (61%), |
| Predicted by PROVEAN: | C: N, D: N, E: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, N: N, P: N, Q: N, R: N, S: N, T: N, V: N, W: N, Y: N, |
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[hide] CD133+ melanoma subpopulations contribute to periv... Cancer Res. 2012 Oct 1;72(19):5111-8. doi: 10.1158/0008-5472.CAN-12-0624. Epub 2012 Aug 3. Lai CY, Schwartz BE, Hsu MY
CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry.
Cancer Res. 2012 Oct 1;72(19):5111-8. doi: 10.1158/0008-5472.CAN-12-0624. Epub 2012 Aug 3., [PMID:22865455]
Abstract [show]
Tumor cell subpopulations that express cancer stem cell markers such as CD133 (prominin1) or ABCB5 are thought to be crucial for tumor initiation and heterogeneity, but their biological significance in melanoma has been controversial. Here, we report that CD133(+) and ABCB5(+) subpopulations are colocalized in melanomas in perivascular niches that contain CD144 (VE-cadherin)(+) melanoma cells forming vessel-like channels, a phenomenon termed vasculogenic mimicry (VM). RNAi-mediated attenuation of CD133 established its critical function in morphogenesis of these perivascular niches as well as in melanoma tumorigenicity. Niche-associated genes CD144 and ABCB5 were downregulated in tumors derived from CD133 knockdown (KD) melanoma cells compared with controls. CD133KD cells also lacked the ability to form CD144(+) VM-like channels in a manner that was associated with a depletion of the ABCB5(+) cell subpopulation. Finally, CD133 KD cells exhibited poorer tumor growth in vivo. Taken together, our findings corroborate models in which CD133(+)/ABCB5(+) melanoma cells reside in a complex anastomosing microvascular niche that encompasses CD144(+) VM channels as well as authentic endothelial cell-lined blood vessels. Further, they indicate that CD133(+) cells act as stem-like cells, which drive tumor growth by promoting VM and the morphogenesis of a specialized perivascular niche in melanoma.
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No. Sentence Comment
69 Results CD133 expression in melanoma correlates with tumor progression in vivo Using flow cytometry, we identified CD133&#fe; fractions in 7 melanoma cell lines, including WM164, WM1617, A375P-5, and Figure 1.
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ABCB5 p.Ala375Pro 22865455:69:187
status: NEW76 Melanoma cell lines WM115 WM239A WM983A WM983C WM164 1205Lu WM1617 A375P-5 C b2;3 integrin CD133 Merged High MW PG CD133 A375 Nevi Primary metastatic Melanoma Patient samples (n = 7) % with CD133 + cells B Merged Neg. Cont. Neg. Cont. 400X 400X 35 4 3 2 1 0 CD133&#fe; Subset in Niche Formation and Melanoma Growth www.aacrjournals.org Cancer Res; 72(19) October 1, 2012 5113 2 isogenic cell pairs: WM115 (VGP)/WM239A (Met) and WM983A (VGP)/WM983C (Met), out of a total of (7/9) in vitro.
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ABCB5 p.Ala375Pro 22865455:76:67
status: NEW