ABCMdb

ABCC1 p.Ala587Val

Predicted by SNAP2:	C: D (59%), D: D (80%), E: D (85%), F: D (80%), G: D (66%), H: D (80%), I: D (71%), K: D (85%), L: D (75%), M: D (59%), N: D (66%), P: D (80%), Q: D (66%), R: D (85%), S: N (97%), T: N (82%), V: D (66%), W: D (85%), Y: D (85%),
Predicted by PROVEAN:	C: N, D: D, E: N, F: D, G: N, H: D, I: D, K: N, L: D, M: D, N: N, P: D, Q: N, R: D, S: N, T: N, V: D, W: D, Y: D,

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Publications
[hide] Sulfadiazine resistance in Toxoplasma gondii: no i... Parasite. 2013;20:19. doi: 10.1051/parasite/2013020. Epub 2013 May 27. Doliwa C, Escotte-Binet S, Aubert D, Sauvage V, Velard F, Schmid A, Villena I
Sulfadiazine resistance in Toxoplasma gondii: no involvement of overexpression or polymorphisms in genes of therapeutic targets and ABC transporters.
Parasite. 2013;20:19. doi: 10.1051/parasite/2013020. Epub 2013 May 27., [PMID:23707894]

Abstract [show]
Several treatment failures have been reported for the treatment of toxoplasmic encephalitis, chorioretinitis, and congenital toxoplasmosis. Recently we found three Toxoplasma gondii strains naturally resistant to sulfadiazine and we developed in vitro two sulfadiazine resistant strains, RH-R(SDZ) and ME-49-R(SDZ), by gradual pressure. In Plasmodium, common mechanisms of drug resistance involve, among others, mutations and/or amplification within genes encoding the therapeutic targets dhps and dhfr and/or the ABC transporter genes family. To identify genotypic and/or phenotypic markers of resistance in T. gondii, we sequenced and analyzed the expression levels of therapeutic targets dhps and dhfr, three ABC genes, two Pgp, TgABC.B1 and TgABC.B2, and one MRP, TgABC.C1, on sensitive strains compared to sulfadiazine resistant strains. Neither polymorphism nor overexpression was identified. Contrary to Plasmodium, in which mutations and/or overexpression within gene targets and ABC transporters are involved in antimalarial resistance, T. gondii sulfadiazine resistance is not related to these toxoplasmic genes studied.

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No. Sentence Comment
58 In the resistant strain TgH 32006, one mutation converting Alanine to Valine at position 587 was found in exon 5 [7]. Login to comment
84 Genotype Sulfadiazine susceptibility TgABC.B1 TgABC.B2 TgABC.C1 dhps dhfr RH I Sensitive * (20) T9A,K1285Q EU213065.1 * (22) S2N, S267N, G368D, I509T, L729M, M848L, L883I EF418617.1 * (19) K7E, D491E, T665S G672E, H954P, G968A E1125A, S1718R V1722A, P1785S FJ201251.1 *(2) E474K, R560K A597E *(1) PRU II Sensitive 0 0 0 0 0 EU213066.1 EF418618.1 FJ201252.1 GQ415579.1 GQ397454.1 NED III Sensitive * (13) 0 0 0 0 T9A,K1285Q EU213067.1 EF418619.1 FJ201253.1 GQ415579.1 GQ397454.1 TgA 103001 I Resistant * (19) T9A, K1285Q FJ201255.1 * (23) S2N, S267N, G368D, I509T, G816E, M848L, L883I FJ201256.1 * (23) K7E, D491E, G782A H954P, G968A E1125A, G1440A, H1659Q, S1718R, V1722A, P1785S FJ201254.1 * (2) E474K, R560K, A597E GQ415574.1 * (1) GQ415574.1 TgH 32006 II Resistant 0 0 0 A587V 0 FJ201257.1 FJ215662.1 GQ865628.1 GQ865628.1 GQ397458.1 TgH 32045 II Variant Resistant * (1) *(1) L729M K642E, H1659Q 0 0 FJ201258.1 GQ865630.1 GQ865629.1 GQ415580.1 GQ397459.1 of expression for the TgABC.B2 gene. Login to comment