ABCC1 p.Asn35Thr
| Predicted by SNAP2: | A: N (72%), C: D (59%), D: N (82%), E: N (78%), F: D (66%), G: N (57%), H: N (82%), I: N (57%), K: N (78%), L: N (53%), M: N (61%), P: D (53%), Q: N (93%), R: N (66%), S: N (82%), T: N (72%), V: N (57%), W: D (80%), Y: D (66%), |
| Predicted by PROVEAN: | A: D, C: D, D: N, E: N, F: D, G: D, H: N, I: D, K: N, L: D, M: D, P: D, Q: N, R: N, S: N, T: D, V: D, W: D, Y: D, |
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[hide] Caspase 3 activation is controlled by a sequence l... Biochem Biophys Res Commun. 2004 Mar 26;316(1):93-9. Pelletier M, Cartron PF, Delaval F, Meflah K, Vallette FM, Oliver L
Caspase 3 activation is controlled by a sequence located in the N-terminus of its large subunit.
Biochem Biophys Res Commun. 2004 Mar 26;316(1):93-9., [PMID:15003516]
Abstract [show]
We report that the induction and completion of the apoptotic program is delayed in a doxorubicin-resistant cell line (HL60/ADR). This hindrance to cell death occurred downstream of the multidrug-resistant protein (mrp), a transmembrane transporter. In vitro studies showed that these cells were incapable of correctly activating procaspase 3 (pC3), the main executioner of apoptosis. Sequencing of HL60/ADR pC3 revealed point mutations in a sequence located in the N-terminal region of the large subunit of caspase 3 (C3, amino acids 31-37; i.e., immediately after the propeptide). We called this particular form of C3, the C3 N-terminal modified (C3-NTM), and show that it is partially active when transfected into MCF-7 cells shown to have little or no endogenous pC3. As a deletion of the amino acids 31-37 in wild-type C3 leads to the same phenotype, we conclude that this sequence is involved in C3 activation during apoptosis.
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No. Sentence Comment
137 On the other hand, the DEVDase activity was significantly decreased by the substitution of a Leu for a Trp at position 33 (L33W, P ¼ 0:0133), by a Ser for a Gly at position 36 (S36G, P ¼ 0:0133) or by that of an Asn for a Thr at position 35 (N35T, P ¼ 0:003).
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ABCC1 p.Asn35Thr 15003516:137:252
status: NEW136 On the other hand, the DEVDase activity was significantly decreased by the substitution of a Leu for a Trp at position 33 (L33W, P &#bc; 0:0133), by a Ser for a Gly at position 36 (S36G, P &#bc; 0:0133) or by that of an Asn for a Thr at position 35 (N35T, P &#bc; 0:003).
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ABCC1 p.Asn35Thr 15003516:136:250
status: NEW