ABCB2 p.Leu156Tyr
| Predicted by SNAP2: | A: N (61%), C: N (72%), D: D (71%), E: D (66%), F: N (72%), G: N (72%), H: N (57%), I: N (82%), K: D (63%), M: N (82%), N: D (53%), P: D (66%), Q: N (53%), R: D (59%), S: N (82%), T: N (66%), V: N (93%), W: D (63%), Y: N (72%), |
| Predicted by PROVEAN: | A: N, C: N, D: D, E: D, F: N, G: D, H: D, I: N, K: D, M: N, N: D, P: D, Q: D, R: D, S: N, T: N, V: N, W: N, Y: N, |
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[hide] A region of conformational variability outside the... J Immunol. 1998 Dec 15;161(12):6745-50. Kuhns ST, Pease LR
A region of conformational variability outside the peptide-binding site of a class I MHC molecule.
J Immunol. 1998 Dec 15;161(12):6745-50., [PMID:9862704]
Abstract [show]
Peptide binding is known to influence the conformation of the surface of class I molecules as detected with mAbs and TCR. A new conformationally sensitive epitope on the mouse class I molecule Kb is defined by mAb AF6-88.5. The recognized structure is affected by amino acid substitutions in any of the three external domains of the class I heavy chain and, in addition, is influenced by the substitution of human for mouse beta2-microglobulin. Interestingly, the epitope for this Ab is not affected by mutations within the peptide-binding cleft or by the nature of the peptide bound. These findings indicate that the effect of a change in one domain of class I can radiate to other parts of the molecule. Furthermore, the existence of conformationally sensitive structures outside of the peptide-binding site suggests the possibility that class I molecules may change their structure in response to binding by receptors and ligands such as the TCR and the coligand CD8. Such structural changes may represent signals that can influence cellular activation events.
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No. Sentence Comment
101 AF6-88.5 binding to spontaneous in vivo class I mutants Spontaneous Mutant Mutation Relative Percent AF6 Binding* Kb None 100 Kbm1 E152A, R155Y, L156Y 111 Kbm3 D77S, K89A 98 Kbm5 Y116F 106† Kbm8 Y22F, M23I, E24S, A30N 101 Kbm10 T163A, V165M, W167S, K173E, N174L 97 * The amount of Kb or mutant on the cell surface as determined with mAb 2813-3.
X
ABCB2 p.Leu156Tyr 9862704:101:145
status: NEW