ABCC8 p.Arg657Ala
| Predicted by SNAP2: | A: N (78%), C: N (82%), D: N (87%), E: N (82%), F: N (66%), G: N (82%), H: N (87%), I: N (72%), K: N (97%), L: N (78%), M: N (82%), N: N (97%), P: N (78%), Q: N (93%), S: N (93%), T: N (93%), V: N (78%), W: N (78%), Y: N (66%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, E: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, N: N, P: N, Q: N, S: N, T: N, V: N, W: N, Y: N, |
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[hide] A region of the sulfonylurea receptor critical for... EMBO J. 2000 Sep 15;19(18):4915-25. Wada Y, Yamashita T, Imai K, Miura R, Takao K, Nishi M, Takeshima H, Asano T, Morishita R, Nishizawa K, Kokubun S, Nukada T
A region of the sulfonylurea receptor critical for a modulation of ATP-sensitive K(+) channels by G-protein betagamma-subunits.
EMBO J. 2000 Sep 15;19(18):4915-25., [PMID:10990455]
Abstract [show]
To determine the interaction site(s) of ATP-sensitive K(+) (K(ATP)) channels for G-proteins, sulfonylurea receptor (SUR2A or SUR1) and pore-forming (Kir6.2) subunits were reconstituted in the mammalian cell line, COS-7. Intracellular application of the G-protein betagamma2-subunits (G(betagamma)(2)) caused a reduction of ATP-induced inhibition of Kir6.2/SUR channel activities by lessening the ATP sensitivity of the channels. G(betagamma)(2) bound in vitro to both intracellular (loop-NBD) and C-terminal segments of SUR2A, each containing a nucleotide-binding domain (NBD). Furthermore, a single amino acid substitution in the loop-NBD of SUR (Arg656Ala in SUR2A or Arg665Ala in SUR1) abolished the G(betagamma)(2)-dependent alteration of the channel activities. These findings provide evidence that G(betagamma) modulates K(ATP) channels through a direct interaction with the loop-NBD of SUR.
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No. Sentence Comment
124 Aiming at identifying the critical amino acid residues on the SUR subunit interacting with Gbg2, conserved basic amino acid residues within these amino acid sequences, Arg656 and Arg657 in SL3, and His1501 and Arg1502 in ST3, were substituted by Ala to generate four mutant SUR2As: SLM1 (Arg656,657Ala), SLM2 (Arg657Ala), SLM3 (Arg656Ala) and STM1 (His1501Ala and Arg1502Ala).
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ABCC8 p.Arg657Ala 10990455:124:310
status: NEW