ABCC8 p.Leu368Ala
| Predicted by SNAP2: | A: N (72%), C: N (87%), D: D (75%), E: D (66%), F: N (93%), G: D (53%), H: D (63%), I: N (97%), K: D (66%), M: N (87%), N: D (66%), P: D (75%), Q: D (59%), R: D (66%), S: N (57%), T: N (61%), V: N (97%), W: D (63%), Y: D (63%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: N, G: D, H: D, I: N, K: D, M: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: N, W: D, Y: D, |
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[hide] A new ER trafficking signal regulates the subunit ... Neuron. 1999 Mar;22(3):537-48. Zerangue N, Schwappach B, Jan YN, Jan LY
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
Neuron. 1999 Mar;22(3):537-48., [PMID:10197533]
Abstract [show]
Proper ion channel function often requires specific combinations of pore-forming alpha and regulatory beta subunits, but little is known about the mechanisms that regulate the surface expression of different channel combinations. Our studies of ATP-sensitive K+ channel (K(ATP)) trafficking reveal an essential quality control function for a trafficking motif present in each of the alpha (Kir6.1/2) and beta (SUR1) subunits of the K(ATP) complex. We show that this novel motif for endoplasmic reticulum (ER) retention/retrieval is required at multiple stages of K(ATP) assembly to restrict surface expression to fully assembled and correctly regulated octameric channels. We conclude that exposure of a three amino acid motif (RKR) can explain how assembly of an ion channel complex is coupled to intracellular trafficking.
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No. Sentence Comment
241 Kir6.2⌬12, Kir6.2⌬14, Kir6.2⌬16, Kir6.2⌬18, R365A, GP366/367AA, LR368/369AA, KR370/371AA, L368A, R369A, R369K, K370A, K370R,An important but poorly understood aspect of ion K370H, K370N, K370Q, K370W, K370I, K370E, R371A, R371K, promoter], 1% dextrose, and 1.5% Sea-KEM agarose, pH adjusted to 6 with phosphoric acid) (Uozumi et al., 1995).RR369/371KK, and S372A were then constructed by ligating in complementary oligonucleotides with appropriate overhangs (Figure 3B [b-d and f]).
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ABCC8 p.Leu368Ala 10197533:241:118
status: NEW