ABCC8 p.Ile89Thr
| Predicted by SNAP2: | A: N (72%), C: N (72%), D: D (71%), E: D (66%), F: N (93%), G: N (53%), H: D (63%), K: D (71%), L: N (97%), M: N (87%), N: D (66%), P: D (75%), Q: D (53%), R: N (61%), S: D (53%), T: N (78%), V: N (97%), W: D (59%), Y: D (53%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: N, G: D, H: D, K: D, L: N, M: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: N, W: D, Y: D, |
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[hide] Molecular epidemiology of malaria in Cameroon. XXV... Am J Trop Med Hyg. 2009 Jul;81(1):13-8. Tahar R, Ringwald P, Basco LK
Molecular epidemiology of malaria in Cameroon. XXVIII. In vitro activity of dihydroartemisinin against clinical isolates of Plasmodium falciparum and sequence analysis of the P. falciparum ATPase 6 gene.
Am J Trop Med Hyg. 2009 Jul;81(1):13-8., [PMID:19556559]
Abstract [show]
The Plasmodium falciparum ATPase 6 (Pfatp6), homolog of sarco-endoplasmic reticulum, calcium-dependent ATPase in malaria parasites, has been proposed to be the main target of artemisinins. Four distinct point mutations (L263E, E431K, A623E, and S769N) have been reported to be associated with artemisinin resistance. The Pfatp6 sequence polymorphism was determined to evaluate the prevalence of these mutations in fresh clinical isolates in Yaounde, Cameroon, and compare sequence data with in vitro response to dihydroartemisinin. Two major haplotypes were observed: the wild-type LEAS (n = 60, 62%) and a single mutant LKAS (n = 35, 36%). These amino acid substitutions did not influence the level of in vitro response to dihydroartemisinin (P > 0.05). Plasmodium falciparum isolates from Cameroon are highly sensitive in vitro to artemisinins. However, the relatively high prevalence of E431K may be a warning signal that warrants a regular monitoring of these molecular markers and/or in vitro activity of artemisinin derivatives.
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59 PFA0310c) and K1 (GeneBank accession no.AB121051).Sequence alignment was performed using MacDNASIS Pro software (Hitachi Software Engineering Co.,Yokohama, Japan).The following amino acid substitutions were analyzed for each isolate: L263E, E431K, A623E, and S769N.The presence of novel amino acid residues was also noted.The amino acid substitution observed in some Thai isolates (I89T) was not studied because it was shown to be unrelated to drug sensitivity.12 RESULTS The in vitro responses to dihydroartemisinin were available for 234 isolates from 2001 through 2006 (n = 90 in 2001-2002, n = 93 in 2003, and n = 51 in 2006).
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ABCC8 p.Ile89Thr 19556559:59:384
status: NEW