ABCC8 p.Pro35Ser
| Predicted by SNAP2: | A: N (61%), C: N (57%), D: D (75%), E: D (63%), F: N (53%), G: D (71%), H: N (61%), I: N (82%), K: D (63%), L: N (61%), M: N (57%), N: N (57%), Q: D (53%), R: D (63%), S: N (61%), T: N (72%), V: N (87%), W: D (80%), Y: N (61%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: D, G: D, H: D, I: D, K: D, L: D, M: D, N: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D, |
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[hide] Superroot, a recessive mutation in Arabidopsis, co... Plant Cell. 1995 Sep;7(9):1405-19. Boerjan W, Cervera MT, Delarue M, Beeckman T, Dewitte W, Bellini C, Caboche M, Van Onckelen H, Van Montagu M, Inze D
Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction.
Plant Cell. 1995 Sep;7(9):1405-19., [PMID:8589625]
Abstract [show]
We have isolated seven allelic recessive Arabidopsis mutants, designated superroot (sur1-1 to sur1-7), displaying several abnormalities reminiscent of auxin effects. These characteristics include small and epinastic cotyledons, an elongated hypocotyl in which the connection between the stele and cortical and epidermal cells disintegrates, the development of excess adventitious and lateral roots, a reduced number of leaves, and the absence of an inflorescence. When germinated in the dark, sur1 mutants did not develop the apical hook characteristic of etiolated seedlings. We were able to phenocopy the Sur1- phenotype by supplying auxin to wild-type seedlings, to propagate sur1 explants on phytohormone-deficient medium, and to regenerate shoots from these explants by the addition of cytokinins alone to the culture medium. Analysis by gas chromatography coupled to mass spectrometry indicated increased levels of both free and conjugated indole-3-acetic acid. sur1 was crossed to the mutant axr2 and the altered-auxin response mutant ctr1. The phenotype of both double mutants was additive. The sur1 gene was mapped on chromosome 2 at 0.5 centimorgans from the gene encoding phytochrome B.
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No. Sentence Comment
255 Transformation of tobacco with the root locus B gene of A. rhizogenes(rolB)under the control of the cauliflower mosaic virus 35s promoter (P35S-roB) results in the development of numerous roots (Cardarelli et al., 1987).
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ABCC8 p.Pro35Ser 8589625:255:139
status: NEW257 However, neither free nor conjugated IAA levels were modified in P35S-rolB-transformed tobacco plantsor protoplasts(Nilsson et al., 1993; Schmülling et al., 1993; Delbarre et al., 1994).
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ABCC8 p.Pro35Ser 8589625:257:65
status: NEW[hide] GDP-D-mannose 3,5-epimerase (GME) plays a key role... Plant J. 2009 Nov;60(3):499-508. Epub 2009 Jul 8. Gilbert L, Alhagdow M, Nunes-Nesi A, Quemener B, Guillon F, Bouchet B, Faurobert M, Gouble B, Page D, Garcia V, Petit J, Stevens R, Causse M, Fernie AR, Lahaye M, Rothan C, Baldet P
GDP-D-mannose 3,5-epimerase (GME) plays a key role at the intersection of ascorbate and non-cellulosic cell-wall biosynthesis in tomato.
Plant J. 2009 Nov;60(3):499-508. Epub 2009 Jul 8., [PMID:19619161]
Abstract [show]
The GDP-D-mannose 3,5-epimerase (GME, EC 5.1.3.18), which converts GDP-d-mannose to GDP-l-galactose, is generally considered to be a central enzyme of the major ascorbate biosynthesis pathway in higher plants, but experimental evidence for its role in planta is lacking. Using transgenic tomato lines that were RNAi-silenced for GME, we confirmed that GME does indeed play a key role in the regulation of ascorbate biosynthesis in plants. In addition, the transgenic tomato lines exhibited growth defects affecting both cell division and cell expansion. A further remarkable feature of the transgenic plants was their fragility and loss of fruit firmness. Analysis of the cell-wall composition of leaves and developing fruit revealed that the cell-wall monosaccharide content was altered in the transgenic lines, especially those directly linked to GME activity, such as mannose and galactose. In agreement with this, immunocytochemical analyses showed an increase of mannan labelling in stem and fruit walls and of rhamnogalacturonan labelling in the stem alone. The results of MALDI-TOF fingerprinting of mannanase cleavage products of the cell wall suggested synthesis of specific mannan structures with modified degrees of substitution by acetate in the transgenic lines. When considered together, these findings indicate an intimate linkage between ascorbate and non-cellulosic cell-wall polysaccharide biosynthesis in plants, a fact that helps to explain the common factors in seemingly unrelated traits such as fruit firmness and ascorbate content.
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No. Sentence Comment
30 RESULTS Ascorbate content, regulation of ascorbate biosynthesis and plant phenotype are altered in GME-silenced plants Using a CaMV 35S promoter-controlled RNAi strategy targeting a DNA fragment common to the two GME genes found in tomato (SlGME1 and SlGME2), we generated 15 independent tomato primary transformants (P35S:SlgmeRNAi lines).
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ABCC8 p.Pro35Ser 19619161:30:318
status: NEW43 Ascorbate content was reduced by 40-60% in young fully expanded leaves and by 20-40% in fruits at 20 days post-anthesis (DPA) from P35S:SlgmeRNAi lines (Figure 2c).
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ABCC8 p.Pro35Ser 19619161:43:131
status: NEW46 The results of transcript expression profiling using TOM1 tomato microarray in the P35S:SlgmeRNAi transgenic and control plants are accessible at http://terry.bordeaux.inra.fr:8080/files/.
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ABCC8 p.Pro35Ser 19619161:46:83
status: NEW59 Gene expression, protein and ascorbate content in P35S:SlgmeRNAi transgenic and control plants.
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ABCC8 p.Pro35Ser 19619161:59:50
status: NEW60 (a) The relative abundances of SlGME mRNAs were determined in young leaves and fruit at 20 DPA in P35S:SlgmeRNAi plants (lines L-66, L-70 and L-108) compared to control plants.
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ABCC8 p.Pro35Ser 19619161:60:98
status: NEW62 (b) Comparison of specific regions of silver-stained two-dimensional gel electrophoresis gels of the proteome of fruit pericarp at 20 DPA from P35S:SlgmeRNAi line L-108 and control plants using 100 lg of proteins (see Table S4).
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ABCC8 p.Pro35Ser 19619161:62:143
status: NEW63 (c) Ascorbate content in young leaves and fruit at 20 DPA from P35S:SlgmeRNAi lines L-66, L-70 and L-108 and control (wild-type) plants.
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ABCC8 p.Pro35Ser 19619161:63:63
status: NEW70 the P35S:SlgmeRNAi L-66 and L-108 lines (Figure 4a).
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ABCC8 p.Pro35Ser 19619161:70:4
status: NEW74 In order to investigate to what extent the growth defect observed in the P35S:SlgmeRNAi plants was related to depletion of the ascorbate pool, we germinated P35S:SlgmeRNAi (L-108) and control seeds on L-ascorbate- or L-galactose-supplemented media.
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ABCC8 p.Pro35Ser 19619161:74:73
status: NEWX
ABCC8 p.Pro35Ser 19619161:74:157
status: NEW76 However, supplementation failed to enhance plant growth, and seedling height and fresh weight remained significantly lower than that of the control (Figure 4b), suggesting that plant growth defects cannot be solely attributed to the reduced ascorbate biosynthetic capacity of the P35S:SlgmeRNAi plants.
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ABCC8 p.Pro35Ser 19619161:76:280
status: NEW77 The GME-silenced lines have increased fragility In addition to the visual phenotypes, P35S:SlgmeRNAi plants appeared to be very fragile in the greenhouse.
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ABCC8 p.Pro35Ser 19619161:77:86
status: NEW83 The water potential of transgenic leaves (L-108) was reduced by 50%, but no significant changes were observed for osmotic P35S:SlgmeRNAi line-108P35S:SlgmeRNAi line-66 Line-108 Line-66 Control 16.2 ± 2.3 9.8 ± 2.1 * 6.3 ± 1.8 * Chlorophyll (mg g FW-1 ) Photosynthesis (nmolCO2 µmol photon-1 ) 1.43 ± 0.16 1.09 ± 0.13 * 1.05 ± 0.21 * Paraquat effect (% chlorophyll loss) 46.5 ± 2.8 67.9 ± 4.6 * 76.5 ± 6.5 * (a) (b) Figure 3.
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ABCC8 p.Pro35Ser 19619161:83:122
status: NEW85 (a) Eight-week-old P35S:SlgmeRNAi plants that were severely affected, with a strong bleaching phenotype.
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ABCC8 p.Pro35Ser 19619161:85:19
status: NEW86 (b) Photosynthesis was assayed in mature leaves from four P35S:SlgmeRNAi plants of each line and the control at several light intensities, and chlorophyll content was measured.
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ABCC8 p.Pro35Ser 19619161:86:58
status: NEW93 Growth of plantlets and fruits of P35S:SlgmeRNAi transgenic and control plants.
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ABCC8 p.Pro35Ser 19619161:93:34
status: NEW94 (a) Plantlets at 30 days after sowing and sections of 20 DPA fruit pericarp from P35S:SlgmeRNAi lines L-66, L-70 and L-108 and the control.
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ABCC8 p.Pro35Ser 19619161:94:81
status: NEW99 (b) Seedlings at 10 days after sowing of P35S:SlgmeRNAi line L-108 and the control on MS medium, or MS supplemented with 1 mM L-galactose (Gal) or 0.25 mM L-ascorbic acid (AsA).
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ABCC8 p.Pro35Ser 19619161:99:41
status: NEW104 Together with data from GC-MS metabolome analyses of 20 DPA fruit indicating that the most significant metabolic changes observed in P35S:SlgmeRNAi plants were linked to central and cell-wall-related metabolism (Table S2), these results suggested that the plant fragility and soft fruit traits are possibly linked to modifications of cell-wall polysaccharides.
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ABCC8 p.Pro35Ser 19619161:104:133
status: NEW105 Modification of non-cellulosic cell-wall polysaccharides Cell-wall composition analyses of the P35S:SlgmeRNAi lines L-66 and L-108 performed on alcohol-insoluble residue (AIR) indicated that wall preparations of both stem and 20 DPA fruit were enriched in mannose and were depleted in galactose relative to control plants (Figure 6 and Table S3).
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ABCC8 p.Pro35Ser 19619161:105:95
status: NEW120 Change in the composition of the polysaccharide constituents of the cell wall in the stem and 20 DPA fruit from control plants and P35S:SlgmeRNAi lines L-66 (squares) and L-108 (diamonds).
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ABCC8 p.Pro35Ser 19619161:120:131
status: NEW129 Fragility of the stem, fruit firmness, water (Ww) and osmotic (Ws) potentials, and turgor pressure (Wp) of the leaf in P35S:SlgmeRNAi transgenic line L-108 and control plants.
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ABCC8 p.Pro35Ser 19619161:129:119
status: NEW144 In the P35S:SlgmeRNAi lines, the significant decrease in GME expression and protein level reduced the capacity of the plant to produce ascorbate (Figure 2) and impaired plant development (Figure 4).
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ABCC8 p.Pro35Ser 19619161:144:7
status: NEW145 Under normal growth conditions, the size of the P35S:SlgmeRNAi plants and fruits correlated perfectly with the ascorbate concentration (Figure 2), the photosynthetic capacity of the plant, and its sensitivity to oxidative stress (Figure 3).
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ABCC8 p.Pro35Ser 19619161:145:48
status: NEW