ABCC7 p.Gly1208Leu
| Predicted by SNAP2: | A: D (59%), C: N (57%), D: D (75%), E: D (75%), F: D (63%), H: D (59%), I: D (63%), K: D (75%), L: D (71%), M: D (63%), N: D (63%), P: D (66%), Q: D (66%), R: D (75%), S: D (53%), T: D (59%), V: D (63%), W: D (75%), Y: D (66%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: D, H: D, I: D, K: D, L: D, M: D, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D, |
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[hide] Inhibition of ATPase, GTPase and adenylate kinase ... Biochem J. 1999 May 15;340 ( Pt 1):227-35. Randak C, Auerswald EA, Assfalg-Machleidt I, Reenstra WW, Machleidt W
Inhibition of ATPase, GTPase and adenylate kinase activities of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator by genistein.
Biochem J. 1999 May 15;340 ( Pt 1):227-35., 1999-05-15 [PMID:10229679]
Abstract [show]
In the presence of ATP, genistein, like the ATP analogue adenosine 5'-[beta,gamma-imido]triphosphate (pp[NH]pA), increases cystic fibrosis transmembrane conductance regulator (CFTR) chloride currents by prolonging open times. As pp[NH]pA is thought to increase CFTR currents by interfering with ATP hydrolysis at the second nucleotide-binding fold (NBF-2), the present study was undertaken to investigate the effects of genistein on a fusion protein comprising maltose-binding protein (MBP) and NBF-2 (MBP-NBF-2). MBP-NBF-2 exhibited ATPase, GTPase and adenylate kinase activities that were inhibited by genistein in a partial non-competitive manner with respect to ATP or GTP. Ki values for competitive and uncompetitive inhibition were respectively 20 microM and 63 microM for ATPase, 15 microM and 54 microM for GTPase, and 46 microM and 142 microM for adenylate kinase. For ATPase activity, genistein reduced Vmax by 29% and Vmax/Km by 77%. Additional evidence for complex-formation between genistein and MBP-NBF-2 was obtained by the detection of genistein-dependent alterations in the CD spectrum of MBP-NBF-2 that were consistent with the formation of a higher-ordered state. Addition of MBP-NBF-2 increased the fluorescence intensity of genistein, consistent with a change to a less polar environment. pp[NH]pA partially eliminated this enhanced fluorescence of genistein. These observations provide the first direct biochemical evidence that genistein interacts with CFTR, thus inhibiting NBF-2 activity, and suggest a similar mechanism for genistein-dependent stimulation of CFTR chloride currents.
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No. Sentence Comment
45 The CFTR cDNA sequence from base 3754 to base 4331 [1], coding for the CFTR protein sequence from Gly-1208 to Leu-1399 (numbering according to [1]), enclosing the whole predicted NBF-2, was introduced between the EcoRI and PstI sites of the expression vector pMAL2-c2 (New England Biolabs2) in-frame with the coding sequence for MBP.
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ABCC7 p.Gly1208Leu 10229679:45:98
status: NEW[hide] Activation of G551D CFTR channel with MPB-91: regu... Am J Physiol Cell Physiol. 2001 Nov;281(5):C1657-66. Derand R, Bulteau-Pignoux L, Mettey Y, Zegarra-Moran O, Howell LD, Randak C, Galietta LJ, Cohn JA, Norez C, Romio L, Vierfond JM, Joffre M, Becq F
Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation.
Am J Physiol Cell Physiol. 2001 Nov;281(5):C1657-66., [PMID:11600430]
Abstract [show]
We have designed and synthesized benzo[c]quinolizinium derivatives and evaluated their effects on the activity of G551D cystic fibrosis transmembrane conductance regulator (CFTR) expressed in Chinese hamster ovary and Fisher rat thyroid cells. We demonstrated, using iodide efflux, whole cell patch clamp, and short-circuit recordings, that 5-butyl-6-hydroxy-10-chlorobenzo[c]quinolizinium chloride (MPB-91) restored the activity of G551D CFTR (EC(50) = 85 microM) and activated CFTR in Calu-3 cells (EC(50) = 47 microM). MPB-91 has no effect on the ATPase activity of wild-type and G551D NBD1/R/GST fusion proteins or on the ATPase, GTPase, and adenylate kinase activities of purified NBD2. The activation of CFTR by MPB-91 is independent of phosphorylation because 1) kinase inhibitors have no effect and 2) the compound still activated CFTR having 10 mutated protein kinase A sites (10SA-CFTR). The new pharmacological agent MPB-91 may be an important candidate drug to ameliorate the ion transport defect associated with CF and to point out a new pathway to modulate CFTR activity.
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No. Sentence Comment
75 A second fusion protein (MBP/NBD2) of the maltose-binding protein (MBP) and the human CFTR protein sequence from Gly-1208 to Leu-1399 (numbering according to Ref. 25), enclosing the whole predicted second nucleotide binding domain of CFTR (NBD2), was expressed, purified, and characterized in Escherichia coli (23, 24); all enzymatic assays were performed as described in Refs. 23 and 24.
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ABCC7 p.Gly1208Leu 11600430:75:113
status: NEW[hide] A recombinant polypeptide model of the second nucl... FEBS Lett. 1997 Jun 30;410(2-3):180-6. Randak C, Neth P, Auerswald EA, Eckerskorn C, Assfalg-Machleidt I, Machleidt W
A recombinant polypeptide model of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator functions as an active ATPase, GTPase and adenylate kinase.
FEBS Lett. 1997 Jun 30;410(2-3):180-6., [PMID:9237625]
Abstract [show]
CFTR-NBF-2 expressed and purified in fusion with the maltose-binding protein was shown to catalyse the reaction ATP-->ADP+Pi by three different assays, monitoring ATP turnover, formation of ADP and release of Pi (Km 86 microM, rate constant 0.37 min(-1)). The reaction product ADP inhibits this ATPase activity. In a similar manner the hydrolysis of GTP to GDP and Pi was demonstrated (Km 40 microM, rate constant 0.29 min(-1)). In the presence of AMP the ATPase reaction was superseded by the formation of two ADP from ATP and AMP. As typical for adenylate kinases a distinct AMP-binding site could be verified for CFTR-NBF-2 by the inability of TNP-ATP and AMP to compete for binding. All three enzymatic activities were inhibited by the symmetric double-substrate-mimicking inhibitor Ap5A. As NBF-2 plays a central role in CFTR channel opening and closing the results reported here are fundamental in understanding mechanisms of CFTR channel activity regulation.
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No. Sentence Comment
31 Overexpression and purification of MBP-NBF-2 fusion proteins The CFTR cDNA sequence from base 3754 to base 4331 [4] coding for the CFTR protein sequence from Gly-1208 to Leu-1399 (numbering according to Ref.
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ABCC7 p.Gly1208Leu 9237625:31:158
status: NEW