ABCC7 p.Gly1208Leu
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PMID: 10229679
[PubMed]
Randak C et al: "Inhibition of ATPase, GTPase and adenylate kinase activities of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator by genistein."
No.
Sentence
Comment
45
The CFTR cDNA sequence from base 3754 to base 4331 [1], coding for the CFTR protein sequence from Gly-1208 to Leu-1399 (numbering according to [1]), enclosing the whole predicted NBF-2, was introduced between the EcoRI and PstI sites of the expression vector pMAL2-c2 (New England Biolabs2) in-frame with the coding sequence for MBP.
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ABCC7 p.Gly1208Leu 10229679:45:98
status: NEW
PMID: 11600430
[PubMed]
Derand R et al: "Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation."
No.
Sentence
Comment
75
A second fusion protein (MBP/NBD2) of the maltose-binding protein (MBP) and the human CFTR protein sequence from Gly-1208 to Leu-1399 (numbering according to Ref. 25), enclosing the whole predicted second nucleotide binding domain of CFTR (NBD2), was expressed, purified, and characterized in Escherichia coli (23, 24); all enzymatic assays were performed as described in Refs. 23 and 24.
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ABCC7 p.Gly1208Leu 11600430:75:113
status: NEW
PMID: 9237625
[PubMed]
Randak C et al: "A recombinant polypeptide model of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator functions as an active ATPase, GTPase and adenylate kinase."
No.
Sentence
Comment
31
Overexpression and purification of MBP-NBF-2 fusion proteins The CFTR cDNA sequence from base 3754 to base 4331 [4] coding for the CFTR protein sequence from Gly-1208 to Leu-1399 (numbering according to Ref.
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ABCC7 p.Gly1208Leu 9237625:31:158
status: NEW