ABCC7 p.Gln1411Asp

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PMID: 23378596 [PubMed] Hunt JF et al: "Cystic fibrosis transmembrane conductance regulator (ABCC7) structure."
No. Sentence Comment
289 Improvements in the yield of soluble protein were obtained by introducing the hydrolytically inactivating H1402A mutation in ATPase active site of hNBD2 plus a series of "solubilizing" mutations on its surface (Q1280E/ Y1307N/W1310H/Q1411D) (X Zhao, S Atwell, JF Hunt, et al., unpubl.).
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ABCC7 p.Gln1411Asp 23378596:289:233
status: NEW
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PMID: 24191035 [PubMed] Bozoky Z et al: "Regulatory R region of the CFTR chloride channel is a dynamic integrator of phospho-dependent intra- and intermolecular interactions."
No. Sentence Comment
117 Q1411D; L1436D; and H1402A) designed by SGX Inc. We find similar patterns of R region binding to NBD2 as for NBD1 (Fig. 1E), identifying NBD2 as a direct binding partner for R region and implying that the NBDs have similar binding properties.
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ABCC7 p.Gln1411Asp 24191035:117:0
status: NEW
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239 The NBD2 domain of human CFTR (aa 1193-1445, Q1280E; Y1307N; Q1411D; H1402A; and L1436D), a construct with solubilizing mutations designed by SGX, Inc., was encoded on a pET-SUMO plasmid (Invitrogen).
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ABCC7 p.Gln1411Asp 24191035:239:61
status: NEW
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