ABCMdb

PMID: 24191035

Bozoky Z, Krzeminski M, Muhandiram R, Birtley JR, Al-Zahrani A, Thomas PJ, Frizzell RA, Ford RC, Forman-Kay JD
Regulatory R region of the CFTR chloride channel is a dynamic integrator of phospho-dependent intra- and intermolecular interactions.
Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4427-36. doi: 10.1073/pnas.1315104110. Epub 2013 Nov 4., [PubMed]

Sentences

No. Mutations Sentence Comment

110 ABCC7 p.Tyr1307Asn ABCC7 p.Gln1280Glu Binding was also probed to human CFTR NBD2 using a construct containing five solubilizing mutations (aa 1193-1445, Q1280E; Y1307N; Fig. 2. Login to comment 117 ABCC7 p.His1402Ala ABCC7 p.Gln1411Asp ABCC7 p.Leu1436Asp Q1411D; L1436D; and H1402A) designed by SGX Inc. We find similar patterns of R region binding to NBD2 as for NBD1 (Fig. 1E), identifying NBD2 as a direct binding partner for R region and implying that the NBDs have similar binding properties. Login to comment 237 ABCC7 p.Phe833Leu ABCC7 p.Phe833Leu The human CFTR R region (aa 654-838; F833L) was prepared as described in the work by Baker et al. (23), and the F833L polymorphism was used to optimize solubility. Login to comment 239 ABCC7 p.His1402Ala ABCC7 p.Tyr1307Asn ABCC7 p.Gln1411Asp ABCC7 p.Gln1280Glu ABCC7 p.Leu1436Asp The NBD2 domain of human CFTR (aa 1193-1445, Q1280E; Y1307N; Q1411D; H1402A; and L1436D), a construct with solubilizing mutations designed by SGX, Inc., was encoded on a pET-SUMO plasmid (Invitrogen). Login to comment 252 ABCC7 p.Ala789Cys Paramagnetic relaxation enhancements were measured on MTSL-labeled 15 N,13 C R region mutant A789C/C833S in 125 mM KH2PO4, pH 6.8, 125 mM KCl, and 2 mM EDTA by measuring HNCO spectra (46) recorded with eight transients at 10 &#b0;C in both oxidized and reduced states. Login to comment