ABCB2 p.Gly645Arg
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PMID: 15897556
[PubMed]
Yang T et al: "A rare transporter associated with antigen processing polymorphism overpresented in HLAlow colon cancer reveals the functional significance of the signature domain in antigen processing."
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In addition, we found that mutations S644R, G645R, G646S, and G646D interfered withTAP-1activity.TAP-1G646D, which showed the most severe defect, resided normally in the endoplasmic reticulum and associated with the peptide loading complex, but failed to transport peptide across the endoplasmic reticulum membrane.
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ABCB2 p.Gly645Arg 15897556:3:44
status: NEW67 Nucleotide changes and amino acid substitutions generated by site-directed mutagenesis Signature motif sequence Nucleotide change Amino acid change LSGGQ TCA >CGA S644R LSGGQ GGG >CGG G645R LSGGQ GGT >GAT G646D LSGGQ GGT >AGT G646S Golden, CO), which were diluted in 200 AL of dilution buffer (PBS containing 3% bovine serum and 0.2% Triton X-100) were added.
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ABCB2 p.Gly645Arg 15897556:67:184
status: NEW149 To substantiate the functional significance of the TAP-1 signature motif, we introduced the following mutations into the signature motif of TAP-1: S644R, G645R, G646S, and G646D (Table 1) by site-directed mutagenesis.
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ABCB2 p.Gly645Arg 15897556:149:154
status: NEW186 A, SK-MEL-19 cell clones stably transfected with theTAP-1mutants, G644R, G645R, G646S, and G646Das indicated were examined for their surface MHC class Iby flow cytometry after being stained with a phycoerythrin-conjugated anti-human HLA-A, B, and C antibody (boldline) or isotype control (phycoerythrin-conjugated mouse IgG1 , dotted lines).
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ABCB2 p.Gly645Arg 15897556:186:73
status: NEW