ABCMdb

PMID: 15897556

Yang T, Lapinski PE, Zhao H, Zhou Q, Zhang H, Raghavan M, Liu Y, Zheng P
A rare transporter associated with antigen processing polymorphism overpresented in HLAlow colon cancer reveals the functional significance of the signature domain in antigen processing.
Clin Cancer Res. 2005 May 15;11(10):3614-23., [PubMed]

Sentences

No. Mutations Sentence Comment

3 ABCB2 p.Gly645Arg In addition, we found that mutations S644R, G645R, G646S, and G646D interfered withTAP-1activity.TAP-1G646D, which showed the most severe defect, resided normally in the endoplasmic reticulum and associated with the peptide loading complex, but failed to transport peptide across the endoplasmic reticulum membrane. Login to comment 67 ABCB2 p.Gly645Arg Nucleotide changes and amino acid substitutions generated by site-directed mutagenesis Signature motif sequence Nucleotide change Amino acid change LSGGQ TCA >CGA S644R LSGGQ GGG >CGG G645R LSGGQ GGT >GAT G646D LSGGQ GGT >AGT G646S Golden, CO), which were diluted in 200 AL of dilution buffer (PBS containing 3% bovine serum and 0.2% Triton X-100) were added. Login to comment 149 ABCB1 p.Ser644Arg ABCB2 p.Gly645Arg To substantiate the functional significance of the TAP-1 signature motif, we introduced the following mutations into the signature motif of TAP-1: S644R, G645R, G646S, and G646D (Table 1) by site-directed mutagenesis. Login to comment 152 ABCB1 p.Ser644Arg To substantiate the functional significance of the TAP-1 signature motif, we introduced the following mutations into the signature motif of TAP-1: S644R, G645R, G646S, and G646D (Table 1) by site-directed mutagenesis. Login to comment 186 ABCB2 p.Gly645Arg A, SK-MEL-19 cell clones stably transfected with theTAP-1mutants, G644R, G645R, G646S, and G646Das indicated were examined for their surface MHC class Iby flow cytometry after being stained with a phycoerythrin-conjugated anti-human HLA-A, B, and C antibody (boldline) or isotype control (phycoerythrin-conjugated mouse IgG1 , dotted lines). Login to comment 217 ABCB1 p.Arg659Gln In fact, in a previous study (19), no cell surface HLA was detectable in a small cell lung cancer cell line due to selective expression of another TAP-1 variant, R659Q, which was later shown to have as much as 50% of wild-type transporter activity (34). Login to comment 220 ABCB1 p.Arg659Gln In fact, in a previous study (19), no cell surface HLA was detectable in a small cell lung cancer cell line due to selective expression of another TAP-1 variant, R659Q, which was later shown to have as much as 50% of wild-type transporter activity (34). Login to comment 296 ABCC7 p.Gly551Asp Derand R, Bulteau-Pignoux L, Becq F.The cystic fibrosis mutation G551D alters the non-Michaelis-Menten behavior of the cystic fibrosis transmembrane conductance regulator (CFTR) channel and abolishes the inhibitory Genistein binding site. Login to comment