ABCB1 p.Tyr307Phe
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PMID: 24366667
[PubMed]
Donmez Cakil Y et al: "Pore-exposed tyrosine residues of P-glycoprotein are important hydrogen-bonding partners for drugs."
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Sentence
Comment
53
Construction of P-gp Mutants The following primers were used for generation of the Y307F, Y310F, Y307F/Y310F, Y950F, Y953F, and Y950F/Y953F mutations of hexa-His-tagged human P-gp in the entry vector pENTR4: Y307F- forward, 59-CTTTCCTGCTGATCTTTGCATCTTATGCTCTGGCC-39; Y307F-reverse, 59-GGCCAGAGCATAAGATGCAAAGATCAGCAGG AAAG-39; Y310F-forward, 59-CTTTCCTGCTGATCTATGCATCTTT TGCTCTGGCC-39; Y310F-reverse, 59-GGCCAGAGCAAAAGATGCA- TAGATCAGCAGGAAAG-39; Y307F/Y310F-forward, 59-CTTTCCTG CTGATCTTTGCATCTTTTGCTCTGGCC-39; Y307F/Y310F-reverse, 59-GGCCAGAGCAAAAGATGCAAAGATCAGCAGGAAAG-39; Y950F- forward, 59-TCACCCAGGCAATGATGTTTTTTTCCTATGCTGGATG- 39; Y950F-reverse, 59-CATCCAGCATAGGAAAAAAACATCATTGCC TGGGTGA-39; Y953F-forward, 59-ACCCAGGCAATGATGTATTTTT CCTTTGCTGGATGTTTC-39; Y953F-reverse, 59-GAAACATCCAGCAAA GGAAAAATACATCATTGCCTGGGT-39; Y950F/Y953F-forward, 59-CCTT CACCCAGGCAATGATGTTTTTTTCCTTTGCTGGATGTTTCC -39; and Y950F/Y953F-reverse, 59-GGAAACATCCAGCAAAGGAAAAAAACATCA TTGCCTGGGTGAAGG-39.
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ABCB1 p.Tyr307Phe 24366667:53:83
status: NEWX
ABCB1 p.Tyr307Phe 24366667:53:97
status: NEWX
ABCB1 p.Tyr307Phe 24366667:53:208
status: NEWX
ABCB1 p.Tyr307Phe 24366667:53:267
status: NEWX
ABCB1 p.Tyr307Phe 24366667:53:445
status: NEWX
ABCB1 p.Tyr307Phe 24366667:53:510
status: NEW114 All mutants were detected at the plasma membrane, although the expression levels of Q132R/Y950F, Q773R/Y950F, and Y307F/Y310F were reduced (Supplemental Fig. 5).
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ABCB1 p.Tyr307Phe 24366667:114:114
status: NEW