ABCB4 p.Asp805Cys
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PMID: 24064216
[PubMed]
Kapoor K et al: "Mutations in intracellular loops 1 and 3 lead to misfolding of human P-glycoprotein (ABCB1) that can be rescued by cyclosporine A, which reduces its association with chaperone Hsp70."
No.
Sentence
Comment
416
Lane 1, cysless-WT; lane 2, D164C/D805C; lane 3, cysless-WT (CsA); lane 4, D164C/D805C (CsA).
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ABCB4 p.Asp805Cys 24064216:416:34
status: NEWX
ABCB4 p.Asp805Cys 24064216:416:81
status: NEW424 Importantly, like èc;F508-CFTR (43), once the D164C/D805C mutant P-gp is rescued and reaches the cell surface, it is functional to the same levels as cysless-WT.
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ABCB4 p.Asp805Cys 24064216:424:56
status: NEW
PMID: 25987565
[PubMed]
Loo TW et al: "The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein."
No.
Sentence
Comment
289
To test if residues Asp-164 or Asp-805 were essential for activity, Kapoor et al. (51) tested the effects of introducing D164C or D805C mutations into human Cys-less P-gp.
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ABCB4 p.Asp805Cys 25987565:289:130
status: NEW291 The D164C mutation reduced cell surface expression to about 40% of the Cys-less parent while the presence of both the D164C and D805C mutations reduced cell surface expression to about 20% of the parent.
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ABCB4 p.Asp805Cys 25987565:291:128
status: NEW292 Immunoblot analysis of whole cell extracts expressing D164C/D805C showed that the major product was immature P-gp.
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ABCB4 p.Asp805Cys 25987565:292:60
status: NEW293 The observation that P-gp mutants R262A/ R905A, T263A/T906A (this study), and D164C/D805C (51) retain substantial activity suggests that there are multiple NBD-TMD contacts as reported by Jin et al. (21).
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ABCB4 p.Asp805Cys 25987565:293:84
status: NEW294 The D164C/D805C mutant could be efficiently rescued when expressed in the presence of cyclosporine A to yield mature P-gp as the major product.
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ABCB4 p.Asp805Cys 25987565:294:10
status: NEW