ABCC1 p.Ile152Leu
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PMID: 18824527
[PubMed]
Tradtrantip L et al: "Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion."
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Sentence
Comment
35
Fisher rat thyroid (FRT) cells expressing human wild-type CFTR and YFP-H148Q/I152L, with or without V2R, were generated as described previously (Yangthara et al., 2007).
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ABCC1 p.Ile152Leu 18824527:35:77
status: NEW45 Transfected FRT cells were plated on black 96-well plates with clear plastic bottoms (Costar; Corning Life Science, Acton, MA), cultured overnight to confluence, washed three times with PBS, and treated with test compounds in a final volume of 60 l. YFP-H148Q/I152L fluorescence was measured using a commercial plate-reader (FluoStar Optima; BMG Labtech, Winooski, VT) equipped with custom excitation and emission filters (500 nm and 544 nm, respectively; Chroma Technology Corp., Brattleboro, VT).
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ABCC1 p.Ile152Leu 18824527:45:268
status: NEW130 A primary screen of 50,000 small molecules was done on FRT cells stably expressing human wild-type V2R and CFTR, and the fluorescent halide sensor YFP-H148Q/I152L.
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ABCC1 p.Ile152Leu 18824527:130:157
status: NEW