ABCMdb

PMID: 18824527

Tradtrantip L, Yangthara B, Padmawar P, Morrison C, Verkman AS
Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion.
Mol Pharmacol. 2009 Jan;75(1):134-42. Epub 2008 Sep 29., [PubMed]

Sentences

No. Mutations Sentence Comment

35 ABCC1 p.Ile152Leu Fisher rat thyroid (FRT) cells expressing human wild-type CFTR and YFP-H148Q/I152L, with or without V2R, were generated as described previously (Yangthara et al., 2007). Login to comment 45 ABCC1 p.Ile152Leu Transfected FRT cells were plated on black 96-well plates with clear plastic bottoms (Costar; Corning Life Science, Acton, MA), cultured overnight to confluence, washed three times with PBS, and treated with test compounds in a final volume of 60 ␮l. YFP-H148Q/I152L fluorescence was measured using a commercial plate-reader (FluoStar Optima; BMG Labtech, Winooski, VT) equipped with custom excitation and emission filters (500 nm and 544 nm, respectively; Chroma Technology Corp., Brattleboro, VT). Login to comment 130 ABCC1 p.Ile152Leu A primary screen of 50,000 small molecules was done on FRT cells stably expressing human wild-type V2R and CFTR, and the fluorescent halide sensor YFP-H148Q/I152L. Login to comment