ABCMdb

ABCC8 p.Ile152Leu

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Publications

PMID: 16787252 [PubMed] Verkman AS et al: "CFTR chloride channel drug discovery--inhibitors as antidiarrheals and activators for therapy of cystic fibrosis."

No. Sentence Comment

209 To identify correctors of defective ∆F508-CFTR gating ('potentiators`), we designed a high-throughput screen using stably transfected FRT cells that co-express ∆F508-CFTR and the halide sensitive indicator YFP-H148Q/I152L [16] (Fig. 6A,B). Login to comment
232 Cells co-expressing ∆F508-CFTR and the halide-sensitive fluorescent protein YFP-H148Q/I152L were grown for 24 h at 27 o C (to allow plasma membrane ∆F508-CFTR expression). Login to comment
233 After washing, test compounds (2.5 µM) and forskolin (20 µM) were added, and I- influx was assayed from the time course of YFP-H148Q/I152L fluorescence after adding Ito the external solution. B. Representative time courses of YFP-H148Q/I152L fluorescence in control wells (saline, negative control; 50 µM genistein, positive control) with examples of inactive and active test compounds. Login to comment

PMID: 21730204 [PubMed] Phuan PW et al: "Cyanoquinolines with independent corrector and potentiator activities restore {delta}phe508-cystic fibrosis transmembrane conductance regulator chloride channel function in cystic fibrosis."

No. Sentence Comment

38 Each of the CFTR-expressing cell lines (and the nontransfected parental cells) was also transfected with halide-sensitive green fluorescent protein YFP-H148Q/I152L/F46L. Login to comment

PMID: 22056837 [PubMed] Saint-Criq V et al: "Azithromycin fails to reduce inflammation in cystic fibrosis airway epithelial cells."

No. Sentence Comment

51 Iodide rather than chloride is used because of strong YFP-H148Q/I152L quenching by iodide, and because CFTR is permeable to iodide (Verkman and Galietta, 2009). Login to comment
107 CFTR activities were determined by CFTR halide conductance in cells expressing an YFP-H148Q/ I152L/F46L indicator stimulated by CFTR agonist mixture of CFTR. Login to comment

PMID: 22973054 [PubMed] Veit G et al: "Proinflammatory cytokine secretion is suppressed by TMEM16A or CFTR channel activity in human cystic fibrosis bronchial epithelia."

No. Sentence Comment

187 Consistent results were obtained by measuring the halide conductance with cytosolic YFP-H148Q/I152L/F46L, a halide-sensitive fluorescent protein (Ferrera et al., 2009; Figure 7, E and F, and Supplemental Figure S5I). Login to comment
355 Halide permeability determination of the PM The halide sensor YFP-F46L/H148Q/I152L (Namkung et al., 2010) was amplified by PCR and cloned into the XhoI/BamHI restriction sites of the pLVX-IRES-Hyg lentiviral vector (Clontech). Login to comment

PMID: 24737137 [PubMed] Phuan PW et al: "Synergy-based small-molecule screen using a human lung epithelial cell line yields DeltaF508-CFTR correctors that augment VX-809 maximal efficacy."

No. Sentence Comment

49 A549 lung epithelial cells (ATCC CCL-185; American Type Culture Collection, Manassas, VA) stably expressing ƊF508-CFTR (Pedemonte et al., 2010) were provided by Dr. Luis Galietta (Genoa, Italy) and cotransfected with halide-sensitive yellow fluorescent protein (YFP)-H148Q/I152L/F46L (Galietta et al., 2001). Login to comment

PMID: 26245207 [PubMed] Phuan PW et al: "Potentiators of Defective DeltaF508-CFTR Gating that Do Not Interfere with Corrector Action."

No. Sentence Comment

30 Fisher rat thyroid (FRT) epithelial cells stably expressing ƊF508, wild-type, or G551D CFTR, together with halide-sensitive green fluorescent protein yellow fluorescence protein (YFP)-H148Q/I152L/F46L (Galietta et al., 2001) were as reported (Ma et al., 2002; Pedemonte et al., 2005). Login to comment