ABCC1 p.Gly756Ser
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PMID: 9379898
[PubMed]
Wemmie JA et al: "Mutational analysis of the Saccharomyces cerevisiae ATP-binding cassette transporter protein Ycf1p."
No.
Sentence
Comment
133
These mutants corresponded to CFTR alterations known to be associated with cystic fibrosis (G551D and G551S in CFTR, G756D and G756S in Ycf1p) as well as lesions that either disturb normal function (K464M in CFTR, K669M in Ycf1p) or act to suppress the phenotype of ⌬F508 CFTR (R553Q and R553M in CFTR, K758Q and K758M in CFTR).
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ABCC1 p.Gly756Ser 9379898:133:127
status: NEW135 K669M, G756S and G756D were unable to complement the cadmium hypersensitivity of the ⌬ycf1 strain. Western blot analysis of these mutant Ycf1p derivatives indicated that all these proteins were produced at the same level in the cell.
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ABCC1 p.Gly756Ser 9379898:135:7
status: NEW237 The three mutant Ycf1p derivatives (⌬F713, G756D and G756S) that correspond to known CF-causing alleles of CFTR (⌬F508, G551D and G551S) all produce a Ycf1p mutant that exhibits a defect analogous to its CFTR counterpart.
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ABCC1 p.Gly756Ser 9379898:237:60
status: NEW281 The 2 m plasmids carrying the indicated mutant forms of YCF1 and the relevant mutagenic PCR primers are listed below: pJAW100 (G756S), 5Ј-ATC TCC TTA TCT GGA TCC CAA AAA GCT CGT TTG-3Ј; pJAW88 (G756D), 5Ј-ATC TCC TTA TCT GGA GAC CAA AAA GCT CGT TTG-3Ј; pJAW86 (K669M), 5Ј- AAA GTT GGC AGT GGT ATG ACA GCT CTA TT-3Ј; pJAW98 (K758R), 5Ј-TTA TCT GGA GGA CAA CGG GCC CGT TTG TCT TTA-3Ј; pJAW108 (K758M), 5Ј-AGA CAA ACG AGC CAT TTG TCC TCC AGA TAA GGA TAT CCC TTT C-3Ј; pJAW109 (K758Q), 5Ј-AGA CAA ACG AGC TTG TTG TCC TCC AGA TAA GCT TAT CCC TTT C-3Ј.
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ABCC1 p.Gly756Ser 9379898:281:135
status: NEW