ABCC4 p.Leu1325Ala
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PMID: 18045536
[PubMed]
Li C et al: "Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia."
No.
Sentence
Comment
159
We synthesized various peptides, with and without a biotin conjugate at the N terminus (MRP4-C10 WT), and mutant peptides with the alanine substitution point mutation of the last amino acid within the tripeptide PDZ motif (MRP4-C10-L1325A) (Figure 7A) and the alanine substitution mutations of the whole tripeptide PDZ motif (MRP4-C10-AAA) (Figure S6A).
X
ABCC4 p.Leu1325Ala 18045536:159:232
status: NEW171 MRP4-L1325A mutant peptide behaved in a similar fashion to the biotin control (Figure S6B).
X
ABCC4 p.Leu1325Ala 18045536:171:5
status: NEW198 MRP4 Peptides that Disrupt MRP4 Interaction with PDZK1 Attenuate Elevation of CFTR ClÀ Currents Mediated by MRP4 Inhibition (A) PDZK1 binding to MRP4 in the presence of biotin-conjugated MRP4 C-tail peptides (last 10 amino acids; wild-type and mutant L1325A).
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ABCC4 p.Leu1325Ala 18045536:198:251
status: NEW253 MRP4 Peptide In Vitro Competitive Binding MRP4 peptides (biotin-conjugated wild-type or L1325A mutant peptide, or nonconjugated wild-type or C10-AAA mutant peptide; 2 mM each) were mixed with His-PDZK1 (1.3 and 6.6 nM) at 22 C-24 C for 1 hr before GST-MRP4-C50 (immobilized on glutathione beads) was added; the solution was then mixed for another hour.
X
ABCC4 p.Leu1325Ala 18045536:253:88
status: NEW158 We synthesized various peptides, with and without a biotin conjugate at the N terminus (MRP4-C10 WT), and mutant peptides with the alanine substitution point mutation of the last amino acid within the tripeptide PDZ motif (MRP4-C10-L1325A) (Figure 7A) and the alanine substitution mutations of the whole tripeptide PDZ motif (MRP4-C10-AAA) (Figure S6A).
X
ABCC4 p.Leu1325Ala 18045536:158:232
status: NEW170 MRP4-L1325A mutant peptide behaved in a similar fashion to the biotin control (Figure S6B).
X
ABCC4 p.Leu1325Ala 18045536:170:5
status: NEW197 MRP4 Peptides that Disrupt MRP4 Interaction with PDZK1 Attenuate Elevation of CFTR Cl Currents Mediated by MRP4 Inhibition (A) PDZK1 binding to MRP4 in the presence of biotin-conjugated MRP4 C-tail peptides (last 10 amino acids; wild-type and mutant L1325A).
X
ABCC4 p.Leu1325Ala 18045536:197:251
status: NEW252 MRP4 Peptide In Vitro Competitive Binding MRP4 peptides (biotin-conjugated wild-type or L1325A mutant peptide, or nonconjugated wild-type or C10-AAA mutant peptide; 2 mM each) were mixed with His-PDZK1 (1.3 and 6.6 nM) at 22 C-24 C for 1 hr before GST-MRP4-C50 (immobilized on glutathione beads) was added; the solution was then mixed for another hour.
X
ABCC4 p.Leu1325Ala 18045536:252:88
status: NEW