ABCMdb

PMID: 18045536

Li C, Krishnamurthy PC, Penmatsa H, Marrs KL, Wang XQ, Zaccolo M, Jalink K, Li M, Nelson DJ, Schuetz JD, Naren AP
Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia.
Cell. 2007 Nov 30;131(5):940-51., [PubMed]

Sentences

No. Mutations Sentence Comment

158 ABCC4 p.Leu1325Ala We synthesized various peptides, with and without a biotin conjugate at the N terminus (MRP4-C10 WT), and mutant peptides with the alanine substitution point mutation of the last amino acid within the tripeptide PDZ motif (MRP4-C10-L1325A) (Figure 7A) and the alanine substitution mutations of the whole tripeptide PDZ motif (MRP4-C10-AAA) (Figure S6A). Login to comment 159 ABCC4 p.Leu1325Ala We synthesized various peptides, with and without a biotin conjugate at the N terminus (MRP4-C10 WT), and mutant peptides with the alanine substitution point mutation of the last amino acid within the tripeptide PDZ motif (MRP4-C10-L1325A) (Figure 7A) and the alanine substitution mutations of the whole tripeptide PDZ motif (MRP4-C10-AAA) (Figure S6A). Login to comment 170 ABCC4 p.Leu1325Ala MRP4-L1325A mutant peptide behaved in a similar fashion to the biotin control (Figure S6B). Login to comment 171 ABCC4 p.Leu1325Ala MRP4-L1325A mutant peptide behaved in a similar fashion to the biotin control (Figure S6B). Login to comment 197 ABCC4 p.Leu1325Ala MRP4 Peptides that Disrupt MRP4 Interaction with PDZK1 Attenuate Elevation of CFTR Cl Currents Mediated by MRP4 Inhibition (A) PDZK1 binding to MRP4 in the presence of biotin-conjugated MRP4 C-tail peptides (last 10 amino acids; wild-type and mutant L1325A). Login to comment 198 ABCC4 p.Leu1325Ala ABCC4 p.Leu1325Ala MRP4 Peptides that Disrupt MRP4 Interaction with PDZK1 Attenuate Elevation of CFTR ClÀ Currents Mediated by MRP4 Inhibition (A) PDZK1 binding to MRP4 in the presence of biotin-conjugated MRP4 C-tail peptides (last 10 amino acids; wild-type and mutant L1325A). Login to comment 252 ABCC4 p.Leu1325Ala MRP4 Peptide In Vitro Competitive Binding MRP4 peptides (biotin-conjugated wild-type or L1325A mutant peptide, or nonconjugated wild-type or C10-AAA mutant peptide; 2 mM each) were mixed with His-PDZK1 (1.3 and 6.6 nM) at 22 C-24 C for 1 hr before GST-MRP4-C50 (immobilized on glutathione beads) was added; the solution was then mixed for another hour. Login to comment 253 ABCC4 p.Leu1325Ala MRP4 Peptide In Vitro Competitive Binding MRP4 peptides (biotin-conjugated wild-type or L1325A mutant peptide, or nonconjugated wild-type or C10-AAA mutant peptide; 2 mM each) were mixed with His-PDZK1 (1.3 and 6.6 nM) at 22 C-24 C for 1 hr before GST-MRP4-C50 (immobilized on glutathione beads) was added; the solution was then mixed for another hour. Login to comment