ABCB9 p.His699Ala
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PMID: 17977821
[PubMed]
Demirel O et al: "Identification of a lysosomal peptide transport system induced during dendritic cell development."
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Comment
47
TAPL-K545A/ H699A was generated by Ligase Chain Reaction using the primers 5Ј-CGGGCAGTGGGGCGAGCTCCTGTGTC-3Ј and 5Ј-CTC- ATCATCGCGGCCCGGCTGAGCAC-3Ј (mutated bases are underlined).
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ABCB9 p.His699Ala 17977821:47:12
status: NEW75 Retroviral Transduction-Recombinant retroviruses were obtained by co-transfection of pCL-Ampho (generous gift of Peter Cresswell) (28), and the retroviral constructs pLPCX- TAPL or pLPCX-TAPL-K545A/H699A into 293T cells with Lipofectamine 2000 (Invitrogen) following the manufacturer`s instructions.
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ABCB9 p.His699Ala 17977821:75:198
status: NEW198 After retroviral transduction, TAPL and the Walker A/H-loop mutant K545A/H699A were detected by immunoblotting as single bands (Fig. 4A).
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ABCB9 p.His699Ala 17977821:198:73
status: NEW213 Perinuclear supernatants (PNS) derivedfromTAPL-wt-orTAPL-K545A/H699A-transducedRajicellswerefrac- tioned by a Percoll gradient and analyzed by SDS-PAGE and immunoblotting.
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ABCB9 p.His699Ala 17977821:213:63
status: NEW